BKV splice sequences based on analysis of preferred donor and acceptor sites.
BKV splice sequences based on analysis of preferred donor and acceptor sites.
复制标题
基于首选供体和受体位点分析的 BKV 剪接序列。
DOI：
10.1093/nar/6.10.3387
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发表时间：
1979
影响因子：
14.9
通讯作者：
R. Dhar
中科院分区：
文献类型：
--
作者：
I. Seif;G. Khoury;R. Dhar
我们已经确定了相应的剪接区域的DNA序列在人类乳多空病毒BKV，SV 40的进化变异体的转录本。为了精确定位BKV序列中的切除点，我们对许多病毒和真核生物的剪接位点序列进行了初步分析。该分析表明，供体位点的首选序列至少属于以下四组之一：Pu↓GTAxG、Pu↓GTAxxT、Pu↓GTxxGT、Pu↓GTxAG（↓ =切割位点）。这四个基团源自两个基本序列Pu↓GTxxG和Pu↓GTA。最佳供体部位可能是：AG↓GTAAGT。受体位点的优选序列为PyPyxPyAG↓形式;间插序列3'端末端AG之前的13个核苷酸内不存在二核苷酸AG。正如我们在BKV剪接位点的研究中所发现的，当DNA序列已知时，优选供体和受体位点的序列在定位RNA剪接点方面提供了预测价值。
We have determined the DNA sequences which correspond to the splicing regions in the transcripts of human papovavirus BKV, an evolutionary variant of SV40. To precisely localize the excision points in the BKV sequence, we have conducted a preliminary analysis of numerous viral and eukaryotic splice site sequences. This analysis suggests that the preferred sequence for the donor site belongs to at least one of four groups: Pu↓GTAxG, Pu↓GTAxxT, Pu↓GTxxGT, Pu↓GTxAG (↓ = the cleavage site). These four groups derive from the two basic sequences, Pu↓GTxxG and Pu↓GTA. An optimal donor site might be: AG↓GTAAGT. The preferred sequence for the acceptor site is of the form PyPyxPyAG↓; no dinucleotide AG occurs within 13 nucleotides prior to the terminal AG of the 3' end of the intervening sequences. As we have found in this study of BKV splice sites, the sequences for the preferred donor and acceptor sites provide predictive value in localizing RNA splice points when the DNA sequence is known.