Growth of Staphylococcus aureus 2064 described by predictive microbiology: From primary to secondary models
Growth of Staphylococcus aureus 2064 described by predictive microbiology: From primary to secondary models
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DOI：
10.2478/acs-2019-0024
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发表时间：
2019-10-01
影响因子：
0.8
通讯作者：
Valik, Lubomir
中科院分区：
文献类型：
--
作者：
Medved'ova, Alzbeta;Havlikova, Adriana;Valik, Lubomir
使用预测微生物学原理和模型，研究了模型营养肉汤中金黄色葡萄球菌 2064 分离株的生长受温度和水分活度的影响。 Baranyi 模型拟合的生长曲线产生的具体速率由次优温度范围 (RTKsub) 的二级 Ratkowsky 模型 (RTKsub) 以及整个温度范围内的 Ratkowsky 扩展模型 (RTKext) 和基数模型 (CM) 建模。以 RTKext 模型的生物学背景为基础，计算并验证了温度 T-min = 6.06 度 G、T-max = 47.9 度 G 和水分活度 a(w) (min )= 0.859 的基值，并通过 CM = 7.72 度 G、T-max = 46.73 度 G、a(wopt) = 0.808）估计的基值进行验证。 CM 还提供了其他基本值，T-opt = 40.63 度 G，a(wopt) = 0.994，以及最佳特定增长率 1.97 h(-1)（在 T-o(pt) 和 a(wopt) 处）。为了评估所有模型的拟合优度，进行了数学和图形验证，统计指标证明了所使用的所有二级模型的适当性。
The growth of Staphylococcus aureus 2064 isolate in model nutrient broth was studied as affected by temperature and water activity using principles and models of predictive microbiology. Specific rates resulting from growth curves fitted by the Baranyi model were modelled by the secondary Ratkowsky model for suboptimal temperature range (RTKsub) as well as the Ratkowsky extended model (RTKext) and cardinal model (CM) in the whole temperature range. With the biological background of the RTKext model, cardinal values of temperature T-min = 6.06 degrees G and T-max = 47.9 degrees G and water activity a(w) (min )= 0.859 were calculated and validated with cardinal values estimated by CM = 7.72 degrees G, T-max = 46.73 degrees G, a(wopt) = 0.808). CM also provided other cardinal values, T-opt = 40.63 degrees G, a(wopt) = 0.994, as well as optimal specific growth rate of 1.97 h(-1) (at T-o(pt) and a(wopt)). To evaluate the goodness of fit of all models, mathematical and graphical validation was performed and the statistical indices proved appropriateness of all the secondary models used.