Screening for Differential Gene Expression During the Development of Form-Deprivation Myopia in the Chicken
Screening for Differential Gene Expression During the Development of Form-Deprivation Myopia in the Chicken
复制标题
DOI：
10.1097/00006324-200402000-00013
复制
发表时间：
2004-02
影响因子：
1.4
通讯作者：
Ian G. Morgan;R. Kucharski;N. Krongkaew;S. I. Firth;P. Megaw;R. Maleszka
中科院分区：
文献类型：
--
作者：
Ian G. Morgan;R. Kucharski;N. Krongkaew;S. I. Firth;P. Megaw;R. Maleszka
目的.应用差异基因显示技术分析形觉剥夺性近视发生和恢复过程中基因表达的变化。方法.采用差异显示-逆转录-聚合酶链反应技术检测配戴散光器诱导形觉剥夺性近视后24 h（包括光照12 h）差异表达的cDNA。信使RNA水平测定定量北方印迹法在视网膜后11天的形式剥夺或视网膜中的扩散器已被删除的前一天。结果在我们的初步筛选中处理了26个差异表达基因。其中两个，B-晶体蛋白和视黄酸受体，进一步研究。B-晶状体蛋白mRNA的水平在第11天增加，在视网膜中的形式剥夺的眼睛相对于对照鸡的眼睛，并在6至12小时内降低到低于这些水平后，删除的扩散。视黄酸受体mRNA的水平显示出类似的变化，除了去除扩散器后，水平进一步增加。结论.差异基因显示技术可用于检测眼生长调控过程中基因表达的变化。B-晶状体蛋白的反应是特别有趣的，因为表达在眼睛生长高时增加，而在眼睛生长减慢时减少。
Purpose. To use the technique of differential gene display to analyze changes in gene expression that occur during the development of and recovery from form-deprivation myopia. Methods. The differential display-reverse transcriptase-polymerase chain reaction technique was used to detect cDNAs that are differentially expressed after 24 h (including 12 h in the light) after fitting with a diffuser to induce form-deprivation myopia. Messenger RNA levels were determined by quantitative Northern blotting in retinas after 11 days of form deprivation or in retinas where the diffusers had been removed the previous day. Results. Twenty-six differentially expressed genes were processed in our initial screen. Two of these, B-crystallin and retinoic acid receptor-, were studied further. Levels of B-crystallin mRNA were increased on day 11 in retinas from form-deprived eyes relative to eyes of control chickens and were reduced to below those levels within 6 to 12 h after removal of the diffusers. Levels of retinoic acid receptor- mRNA showed similar changes, except that after removal of the diffusers, the levels further increased. Conclusions. The technique of differential gene display can be used to detect changes in gene expression during the regulation of eye growth. The response of B-crystallin is particularly interesting because expression increases when eye growth is high and decreases when eye growth slows.