A highly sensitive electrochemical immunosensor based on electrospun nanocomposite for the detection of parathion
A highly sensitive electrochemical immunosensor based on electrospun nanocomposite for the detection of parathion
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DOI：
10.1016/j.foodchem.2022.134371
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发表时间：
2022-10-14
期刊：
影响因子：
8.8
通讯作者：
Wen, Peng
中科院分区：
文献类型：
--
作者：
Yin, Wen-jia;Zhang, Jing-xian;Wen, Peng
为了监测食品中对硫磷农药的残留，构建了一种基于交联聚乙烯醇/柠檬酸纳米纤维膜(PVA/CA NFM)和辣根过氧化物酶标记的抗对硫磷纳米体的灵敏可靠的电化学免疫传感器。首先，制备了具有超高比表面积和均匀形态的交联型PVA/CA NFM并进行了表征。然后，组装了免疫传感器，并对其分析性能进行了评价。该方法对对硫磷具有较高的特异性和敏感性，线性范围为0.01~100 ng/mL，检出限为2.26 pg/mL。此外，该传感器在再生4次后仍保持了近75%的初始活性，储存9周后仍保持85%的活性。食品样品的平均回收率为96.20%~114.61%，变异系数为1.06%~5.28%，与超高效液相色谱测定结果具有良好的相关性(R2=0.9964)。因此，该传感器是一种可行的检测对硫磷的灵敏方法。
For monitoring of the residual of parathion pesticide in food, herein, a sensitive and reliable electrochemical immunosensor based on cross-linked polyvinyl alcohol/citric acid nanofiber membrane (PVA/CA NFM) and horseradish peroxidase labeled anti-parathion nanobody was constructed. Firstly, the cross-linked PVA/CA NFM with extra-high surface area and uniform morphology was prepared and characterized. Then, the immunosensor was assembled and its analytical performances were evaluated. It exhibited high specificity and sensitivity to parathion with the liner range and limit of detection being 0.01-100 ng/mL and 2.26 pg/mL, respectively. Moreover, the biosensor kept almost 75% of its initial activity after regenerating 4 times, and remained 85% after 9 weeks of storage. Finally, the average recoveries from food samples were 96.20%-114.61% with the coefficient of variation being 1.06%-5.28%, which was correlate well with UPLC (R2 = 0.9964). Therefore, the sensor was demonstrated to be a feasible alternative for sensitive assay of parathion.