CD45 cell surface antigens are linked to stimulation of early human myeloid progenitor cells by interleukin 3 (IL-3), granulocyte/macrophage colony-stimulating factor (GM-CSF), a GM-CSF/IL-3 fusion protein, and mast cell growth factor (a c-kit ligand).
CD45 cell surface antigens are linked to stimulation of early human myeloid progenitor cells by interleukin 3 (IL-3), granulocyte/macrophage colony-stimulating factor (GM-CSF), a GM-CSF/IL-3 fusion protein, and mast cell growth factor (a c-kit ligand).
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DOI：
10.1084/jem.174.2.447
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发表时间：
1991-08-01
影响因子：
15.3
通讯作者：
Shen, F W
中科院分区：
文献类型：
--
作者：
Broxmeyer, H E;Lu, L;Hangoc, G;Cooper, S;Hendrie, P C;Ledbetter, J A;Xiao, M;Williams, D E;Shen, F W
CD45抗原是蛋白质酪氨酸磷酸酶。评估了人骨髓祖细胞 (MPC)（集落形成单位粒细胞/巨噬细胞 [CFU-GM]、突发形成单位红细胞 [BFU-E] 和集落形成单位粒细胞/红细胞/巨噬细胞/巨核细胞 [CFU-GEMM]）上 CD45 抗原的表达与集落刺激因子 (CSF)（白细胞介素）对 MPC 的调节之间可能存在的联系。 3 [IL-3]、GM-CSF、G-CSF、M-CSF 和促红细胞生成素 [Epo]）、GM-CSF/IL-3 融合蛋白和肥大细胞生长因子（MGF；c-kit 配体）。用CD45基因外显子1和2而非4、5或6的反义寡脱氧核苷酸（寡核苷酸）或单克隆抗CD45处理细胞，显着减少用GM-CSF、IL-3、融合蛋白和GM-CSF + MGF刺激的CFU-GM集落形成，但用G-CSF或M-CSF刺激则不然。它还减少了 GM-CSF、IL-3、融合蛋白和 MGF 增强的 Epo 依赖性 BFU-E 和 CFU-GEMM 集落形成，但对单独 Epo 刺激的 BFU-E 或 CFU-GEMM 集落形成影响很小或没有影响。使用未分离或纯化的（大于或等于两个细胞之一是 MPC）骨髓细胞获得了类似的结果。由 90% MPC 组成的 CD343+ HLA-DR+ 骨髓细胞的分类群体用于证明交联方案后 CD45 的加帽。此外，用 CD45 基因外显子 1 的反义寡核苷酸处理柱分离的 CD34+ 细胞后，CD45+ 细胞百分比和 CD45 抗原密度下降。这些结果证明 CD45+ 细胞表面抗原与 IL-3、GM-CSF、GM-CSF/IL-3 融合蛋白和 MGF 对早期人类 MPC 的刺激有关。
CD45 antigens are protein tyrosine phosphatases. A possible link was evaluated between expression of CD45 antigens on human myeloid progenitor cells (MPC) (colony-forming unit-granulocyte/macrophage [CFU- GM], burst-forming unit-erythroid [BFU-E], and colony-forming unit- granulocyte/erythroid/macrophage/megakaryocyte [CFU-GEMM]) and regulation of MPC by colony-stimulating factors (CSF) (interleukin 3 [IL-3], GM-CSF, G-CSF, M-CSF, and erythropoietin [Epo]), a GM-CSF/IL-3 fusion protein, and mast cell growth factor (MGF; a c-kit ligand). Treatment of cells with antisense oligodeoxynucleotides (oligos) to exons 1 and 2, but not 4, 5, or 6, of the CD45 gene, or with monoclonal anti-CD45, significantly decreased CFU-GM colony formation stimulated with GM-CSF, IL-3, fusion protein, and GM-CSF + MGF, but not with G-CSF or M-CSF. It also decreased GM-CSF, IL-3, fusion protein, and MGF- enhanced Epo-dependent BFU-E and CFU-GEMM colony formation, but had little or no effect on BFU-E or CFU-GEMM colony formation stimulated by Epo alone. Similar results were obtained with unseparated or purified (greater than or equal to one of two cells being a MPC) bone marrow cells. Sorted populations of CD343+ HLA-DR+ marrow cells composed of 90% MPC were used to demonstrate capping of CD45 after crosslinking protocols. Also, a decreased percent of CD45+ cells and CD45 antigen density was noted after treatment of column-separated CD34+ cells with antisense oligos to exon 1 of the CD45 gene. These results demonstrate that CD45 cell surface antigens are linked to stimulation of early human MPC by IL-3, GM-CSF, a GM-CSF/IL-3 fusion protein, and MGF.