Laboratory diagnosis of two scrub typhus outbreaks at Camp Fuji, Japan in 2000 and 2001 by enzyme-linked immunosorbent assay, rapid flow assay, and Western blot assay using outer membrane 56-kD recombinant proteins
Laboratory diagnosis of two scrub typhus outbreaks at Camp Fuji, Japan in 2000 and 2001 by enzyme-linked immunosorbent assay, rapid flow assay, and Western blot assay using outer membrane 56-kD recombinant proteins
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DOI：
10.4269/ajtmh.2003.69.60
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发表时间：
2003-07-01
影响因子：
3.3
通讯作者：
Richards, AL
中科院分区：
文献类型：
--
作者：
Jiang, J;Marienau, KJ;Richards, AL
2000年10月25日至11月3日和2001年10月17日至11月30日，在日本富士营训练的美国海军陆战队发生了两次恙虫病暴发。2000年约800名海军陆战队员中有9例，2001年约900名海军陆战队员中有8例（发病率分别约为1.1%和0.9%），报告有发热、皮疹、头痛、淋巴结病、肌痛和焦痂的体征和症状。血清学和对强力霉素治疗的快速反应表明他们患有恙虫病。采用酶联免疫吸附试验（ELISA）、快速流动试验（RFA）和免疫印迹试验，对64份来自美国海军陆战队在富士营地训练的恢复期血清样本（18份疑似病例和46份阴性对照）进行了恙虫病东方体（恙虫病病原体）感染证据的评估。除一例疑似病例外，所有病例均有恙虫病血清学证据，所有46份对照血清对O。恙虫病抗原从O. Karp株、Kato株和Gilliam株中获得重组56-kD抗原r56。ELISA形式的恙虫病提供了比单独的Karp r56（ELISA和RFA）或来自Karp、Kato和Gilliam的全细胞抗原制剂（ELISA）更好的结果。
Two scrub typhus outbreaks occurred among U.S. Marines training at Camp Fuji, Japan, between October 25 and November 3, 2000 and October 17 and November 30, 2001. Nine cases in approximately 800 Marines in 2000 and eight cases in approximately 900 Marines in 2001 (approximate attack rates = 1.1% and 0.9%, respectively) reported with signs and symptoms of fever, rash, headache, lymphadenopathy, myalgia, and eschar. Serologies and rapid response to doxycycline treatment indicated they had scrub typhus. Sixty-four convalescent serum samples (18 suspected cases and 46 negative controls) from U.S. Marines training at Camp Fuji during the outbreaks were assessed by enzyme-linked immunosorbent assay (ELISA), rapid flow assay (RFA), and Western blot assay for evidence of infection with Orientia tsutsugamushi, the causative agent of scrub typhus. All but one suspected case had serologic evidence of scrub typhus and all 46 control sera were non-reactive to O. tsutsugamushi antigens. The recombinant 56-kD antigen (r56) from the Karp, Kato and Gilliam strains of O. tsutsugamushi in an ELISA format provided better results than Karp r56 alone (ELISA and RFA) or whole cell antigen preparation from Karp, Kato and Gilliam (ELISA).