Efficient Newly Designed Primers for the Amplification and Sequencing of Bird Mitochondrial Genomes
Efficient Newly Designed Primers for the Amplification and Sequencing of Bird Mitochondrial Genomes
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DOI：
10.1271/bbb.120819
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发表时间：
2013-03-01
影响因子：
1.6
通讯作者：
Shobrak, Mohammed
中科院分区：
文献类型：
--
作者：
Amer, Sayed A. M.;Ahmed, Mohamed Mohamed;Shobrak, Mohammed
本研究从Genbank数据库中收集了27个不同鸟类超目的线粒体基因组，并对它们的基因进行了比对。从比对中，选择保守序列设计新的保守引物用于不同线粒体基因的扩增和测序。这些引物的重复性进行了测试，在不同的鸟类超目的线粒体基因组的扩增和测序，并得到证实。这种方法有助于设计一套新的引物，以加速鸟类有丝分裂基因组的扩增和测序。它还有助于建立有丝分裂基因组标记来研究特有鸟类的遗传框架，作为其保护管理的初步策略。
In the present study, 27 mitochondrial genomes of diverse avian supra-orders were collected from the Genbank database and their genes were aligned separately. From the alignments, the conserved sequences were selected to design novel conserved primers for amplification and sequencing of the different mitochondrial genes. The reproducibility of these primers was tested in the amplification and sequencing of diverse avian supra-order mitochondrial genomes and was confirmed. This method helped in designing a new set of primers to accelerate both the amplification and the sequencing of bird mitogenomes. It also aids in building mitogenome markers in studying the genetic framework of endemic birds as a preliminary strategy for conservation management of them.