Encapsulation in alginate-coated gelatin microspheres improves survival of the probiotic Bifidobacterium adolescentis 15703T during exposure to simulated gastro-intestinal conditions
Encapsulation in alginate-coated gelatin microspheres improves survival of the probiotic Bifidobacterium adolescentis 15703T during exposure to simulated gastro-intestinal conditions
复制标题
DOI：
10.1016/j.foodres.2007.11.001
复制
发表时间：
2008-01-01
影响因子：
8.1
通讯作者：
Hansen, L. Truelstrup
中科院分区：
文献类型：
--
作者：
Annan, N. T.;Borza, A. D.;Hansen, L. Truelstrup
制备藻酸盐包被的明胶微球以包封益生菌双歧杆菌15703 T，目的是在暴露于胃肠道的不利条件期间提高存活率。明胶微球与无细胞毒性的京尼平交联，并涂有由外部或内部来源的Ca 2+交联的藻酸盐。藻酸盐涂层防止胃蛋白酶诱导的明胶微球在模拟胃液（pH 2.0，2 h）中的降解，由于完整微球的缓冲作用，导致显著（P < 0.05）更高的存活数。在模拟胃液（1 h）和肠液（pH 7.4，4 h）中依次孵育后，通过内部和外部Ca 2+源方法，藻酸盐涂层微球的存活细胞数分别为7.6和7.4 log cfu ml（-1），而未涂层明胶微球和游离细胞的存活细胞数分别为6.7和6.4 log cfu ml（-1）。本研究提出了一种新的微胶囊化方法，该方法在暴露于不利的环境条件下保护益生菌。(C)2007爱思唯尔有限公司保留所有权利。
Alginate-coated gelatin microspheres were produced to encapsulate the probiotic Bifidobacterium adolescentis 15703T with the objective of enhancing survival during exposure to the adverse conditions of the gastro-intestinal tract. Gelatin microspheres were cross-linked with the non-cytotoxic genipin and coated with alginate cross-linked by Ca2+ from external or internal sources. The alginate coat prevented pepsin-induced degradation of the gelatin microspheres in simulated gastric juice (pH 2.0, 2 h), resulting in significantly (P < 0.05) higher numbers of survivors due to the buffering effect of intact microspheres. After sequential incubation in simulated gastric (I h) and intestinal juices (pH 7.4, 4 h), number of surviving cells were 7.6 and 7.4 log cfu ml(-1) for alginate coated microspheres by the internal and external Ca2+-source methods, respectively, while 6.7 and 6.4 log cfu ml(-1) were obtained for cells in uncoated gelatin microspheres and free cells, respectively. This study presents a novel microencapsulation method, which protects probiotic bifidobacteria during exposure to adverse environmental conditions. (C) 2007 Elsevier Ltd. All rights reserved.