Stimulation of matrix metalloproteinases by black-pigmented Bacteroides in human pulp and periodontal ligament cell cultures
Stimulation of matrix metalloproteinases by black-pigmented Bacteroides in human pulp and periodontal ligament cell cultures
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DOI：
10.1097/00004770-200202000-00010
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发表时间：
2002-02-01
影响因子：
4.2
通讯作者：
Hsieh, YS
中科院分区：
文献类型：
--
作者：
Chang, YC;Lai, CC;Hsieh, YS
基质金属蛋白酶（MMPs）是一组蛋白水解酶，能够降解细胞外基质的大部分成分。最近，有证据表明MMPs可能在炎症牙髓的组织降解中起作用。迄今为止，关于细菌感染部位细胞外基质破坏的机制知之甚少。本研究的目的是研究牙髓卟啉单胞菌和牙龈卟啉单胞菌的上清液对体外培养的人牙髓和牙周韧带（PDL）细胞产生和分泌MMPs的影响。通过长期培养的底物凝胶酶谱法对结果进行评估。人牙髓和PDL细胞分泌的主要明胶酶在72 kDa处迁移，代表MMP-2。在与MMP-9对应的92 kDa区域也观察到轻微的明胶溶解带。经过8天的培养，发现牙髓假牙霉和牙龈假牙霉在人牙髓和PDL细胞培养中都能提高MMP-2的产生。此外，刺激具有剂量和时间依赖性。然而，用牙髓假牙或牙龈假牙处理的人牙髓和PDL细胞对细胞提取物或条件培养基中产生或分泌的MMP-9的模式没有影响。这些结果表明，黑色拟杆菌在牙髓和根尖周围疾病的组织破坏和细胞外基质崩解中起重要作用。因此，MMPs的激活可能是微生物诱导的牙髓和根尖周围病变发病机制中不同的宿主降解途径之一。了解这些黑色色素拟杆菌种类对牙髓和PDL细胞的作用可能会产生新的治疗方法，以加强目前对牙髓和根尖周围病变的治疗。
Matrix metalloproteinases (MMPs) are a group of proteolytic enzymes capable of degrading most components of the extracellular matrix. Recently, evidence has shown that MMPs may play a role in tissue degradation in inflamed dental pulp. To date very little is known regarding the mechanism of extracellular matrix destruction at the site of bacterial infection. The purpose of this study was to determine the effects of the supernatants from Porphyromonas endodontalis and Porphyromonas gingivalis on the production and secretion of MMPs by primary human pulp and periodontal ligament (PDL) cell cultures in vitro. The results were evaluated by substrate gel zymography from long-term cultures. The main gelatinase secreted by human pulp and PDL cells migrated at 72 kDa and represented MMP-2. Minor gelatinolytic bands were also observed at 92 kDa regions that correspond to MMP-9. After an 8-day culture period, P. endodontalis and P. gingivalis were found to elevate MMP-2 production both in human pulp and PDL cell cultures. In addition, the stimulation was in a dose- and time-dependent manner. Both human pulp and PDL cells, however, treated with either P. endodontalis or P. gingivalis had no effect on the pattern of MMP-9 produced or secreted in either cell extracts or conditioned medium fractions. These results indicate that black-pigmented Bacteroides species play an important role in tissue destruction and disintegration of extracellular matrix in pulpal and periapical diseases. Thus, activation of MMPs may be one of the distinct host degradative pathways in the pathogenesis of microbial-induced pulpal and periapical lesion. An understanding of the actions of these black-pigmented Bacteroides species on pulp and PDL cells may result in new therapies to augment current treatment of pulpal and periapical lesions.