The role of enoyl-CoA hydratase short chain 1 and peroxiredoxin 3 in PP2-induced apoptosis in human breast cancer MCF-7 cells
The role of enoyl-CoA hydratase short chain 1 and peroxiredoxin 3 in PP2-induced apoptosis in human breast cancer MCF-7 cells
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DOI：
10.1016/j.febslet.2010.06.002
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发表时间：
2010-07-16
期刊：
影响因子：
3.5
通讯作者：
Du, Liying
中科院分区：
文献类型：
--
作者：
Liu, Xiang;Feng, Renqing;Du, Liying
我们发现4-氨基-5-（4-氯苯基）-7-（叔丁基）吡唑并[3，4-d]嘧啶（PP 2）诱导人乳腺癌MCF-7细胞凋亡并下调烯酰辅酶A水合酶短链1（ECHS 1）和过氧化物氧还蛋白3（PRDX 3）的表达。Western blot和实时荧光定量RT-PCR证实ECHS 1和PRDX 3在MCF-7和其他癌细胞中的减少。ECHS 1和/或PRDX 3的敲低和过表达进一步支持ECHS 1和PRDX 3在调节PP 2诱导的细胞凋亡中的关键作用。这些结果表明，一个可能的凋亡途径，ECHS 1和PRDX 3的下调增强PP 2诱导的MCF-7细胞凋亡。(C)2010年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
We show that 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2) induces apoptosis and down-regulates the expression of enoyl-CoA hydratase short chain 1 (ECHS1) and peroxiredoxin 3 (PRDX3) in human breast cancer MCF-7 cells. The decrease of ECHS1 and PRDX3 was validated by Western blot and quantitative real-time reverse transcription-PCR in MCF-7 and other carcinoma cells. Knockdown and over-expression of ECHS1 and/or PRDX3 further supported the key role of ECHS1 and PRDX3 in regulation of PP2-induced apoptosis. These results suggest a possible apoptotic pathway whereby down-regulation of ECHS1 and PRDX3 potentiates PP2-induced apoptosis in MCF-7 cells. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.