Human Chorioretinal Layer Thicknesses Measured in Macula-wide, High-Resolution Histologic Sections
Human Chorioretinal Layer Thicknesses Measured in Macula-wide, High-Resolution Histologic Sections
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DOI：
10.1167/iovs.10-6377
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发表时间：
2011-06-01
影响因子：
4.4
通讯作者：
Spaide, Richard F.
中科院分区：
文献类型：
--
作者：
Curcio, Christine A.;Messinger, Jeffrey D.;Spaide, Richard F.
目的。为了全面描述正常人黄斑的脉络膜视网膜层厚度，包括在一些光学相干断层扫描(OCT)设备中能够产生组合信号的两层对(神经节细胞[GCL]和内丛[IPL]层以及外丛[OPL]和外核[ONL]层)。方法：在18名捐献者(年龄40-92岁)黄斑中心凹的0.8亩厚的黄斑区切片上，由一名训练有素的观察者在25个位置测量了21层，并由第二名观察者进行了验证。通过比较体外OCT的视网膜总厚度和切片来评估组织体积的变化。结果：总体组织收缩的中位数为14.5%，中心凹的组织收缩中位数为29%。组织学上的板层边界与SD-OCT扫描中的相似，但黄斑OPL和ONL的形状不同。组织学上GCL、IPL和OPLHenle最厚，为0.8。偏心距分别为1、1.5和0.4 mm。ONL最厚，在中心凹中心向内隆起。偏心距1 mm时，GCL、INL和OPLHenle分别占总视网膜厚度的17.3%~21.1%、18.0%~18.5%和14.2%~16.6%。70岁组RPE和脉络膜厚度分别比70岁组薄17.1%和29.6%，OPLHenle厚20.8%。结论首次建立了正常黄斑脉络膜视网膜层的图形表示和厚度数据库。较新的OCT系统可以区分GCL和IPL，OPLHenle和ONL，OPLHenle在OCT中所占的视网膜厚度比例与组织学有很好的一致性。老年眼OPLHenle增厚可能反映视杆细胞肥大与视杆丢失相关。(投资眼科VS科学。网址：10.1167/iovs.10-6377
PURPOSE. To provide a comprehensive description of chorioretinal layer thicknesses in the normal human macula, including two-layer pairs that can produce a combined signal in some optical coherence tomography (OCT) devices (ganglion cell [GCL] and inner plexiform [IPL] layers and outer plexiform [OPL] and outer nuclear [ONL] layers).METHODS. In 0.8-mu m-thick, macula-wide sections through the foveola of 18 donors (age range, 40-92 years), 21 layers were measured at 25 locations by a trained observer and validated by a second observer. Tissue volume changes were assessed by comparing total retinal thickness in ex vivo OCT and in sections.RESULTS. Median tissue shrinkage was 14.5% overall and 29% in the fovea. Histologic laminar boundaries resembled those in SD-OCT scans, but the shapes of the foveolar OPL and ONL differed. Histologic GCL, IPL, and OPLHenle were thickest at 0.8. to 1, 1.5, and 0.4 mm eccentricity, respectively. ONL was thickest in an inward bulge at the foveal center. At 1 mm eccentricity, GCL, INL, and OPLHenle represented 17.3% to 21.1%, 18.0% to 18.5%, and 14.2% to 16.6% of total retinal thickness, respectively. In donors >= 70 years of age, the RPE and choroid were 17.1% and 29.6% thinner and OPLHenle was 20.8% thicker than in donors < 70 years.CONCLUSIONS. In this study, the first graphic representation and thickness database of chorioretinal layers in normal macula were generated. Newer OCT systems can separate GCL from IPL and OPLHenle from ONL, with good agreement for the proportion of retinal thickness occupied by OPLHenle in OCT and histology. The thickening of OPLHenle in older eyes may reflect Muller cell hypertrophy associated with rod loss. (Invest Ophthalmol Vis Sci. 2011;52:3943-3954) DOI:10.1167/iovs.10-6377