Evidence against singlet oxygen formation by sonolysis of aqueous oxygen-saturated solutions of Hematoporphyrin and Rose Bengal - The mechanism of sonodynamic therapy
Evidence against singlet oxygen formation by sonolysis of aqueous oxygen-saturated solutions of Hematoporphyrin and Rose Bengal - The mechanism of sonodynamic therapy
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DOI：
10.1016/s1350-4177(99)00042-5
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发表时间：
2000-07-01
影响因子：
8.4
通讯作者：
Riesz, P
中科院分区：
文献类型：
--
作者：
Miyoshi, N;Igarashi, T;Riesz, P
单线态氧在声动力学治疗机制中的可能作用，超声和某些声增敏剂的协同作用，进行了研究。我们用4，4 ′-双（1-对羧基苯基-3-甲基-5-羟基）-吡唑（DRD 156），一种灵敏的新试剂，它只与单线态氧（O-1（2））反应，而不与OH自由基、超氧阴离子自由基或H_2O_2反应，产生EPR可检测信号。血卟啉或虎红的90%D2O氧饱和PBS溶液的超声分解（48 kHz）未导致形成DRD 156的半醌自由基的可检测EPR信号。与此相反，半醌自由基的DRD 156的EPR信号观察血卟啉在505 nm或虎红在544 nm的光照射过程中，这些结果是不一致的单线态氧的形成在这些化合物的水溶液的超声分解的主要作用。另一种机制声动力学治疗涉及过氧化氢和烷氧基自由基进行了讨论。(C)出版社：Elsevier Science B. V.
The possible role of singlet oxygen in the mechanism of sonodynamic therapy, the synergistic effect of ultrasound and certain sonosensitizers, was investigated. We used 4,4'-bis(1-p-carboxyphenyl-3-methyl-5-hydroxyl)-pyrazole (DRD 156), a sensitive new reagent which reacts specifically with singlet oxygen (O-1(2)) but not with OH radicals, superoxide anion radicals or H2O2, to produce an EPR detectable signal. Sonolysis (48 kHz) of 90% D2O oxygen-saturated PBS solutions of Hematoporphyrin or Rose Bengal did not lead to the formation of detectable EPR signals of the semiquinone radical of DRD156. In contrast, the EPR signal of the semiquinone radical of DRD156 was observed during photoirradiation of Hematoporphyrin at 505 nm or of Rose Bengal at 544 nm, These results are inconsistent with a major role for singlet oxygen formation in the sonolysis of aqueous solutions of these compounds. An alternative mechanism for sonodynamic therapy involving peroxyl and alkoxyl radicals is discussed. (C) 2000 Published by Elsevier Science B.V.