Quinolone antibiotics induce shiga toxin-encoding bacteriophages, toxin production, and death in mice
Quinolone antibiotics induce shiga toxin-encoding bacteriophages, toxin production, and death in mice
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DOI：
10.1086/315239
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发表时间：
2000-02-01
影响因子：
6.4
通讯作者：
Acheson, DWK
中科院分区：
文献类型：
--
作者：
Zhang, XP;McDaniel, AD;Acheson, DWK
产滋贺毒素大肠杆菌（STEC）引起重大疾病;治疗是支持性的，抗生素的使用是有争议的。环丙沙星而不是磷霉素诱导编码滋贺毒素的噬菌体，并增强大肠杆菌产生滋贺毒素（Stx）。coliO157：H7。在大肠杆菌定殖的小鼠中检查了这一点的潜在临床相关性。大肠杆菌O 157：H7，给予环丙沙星或磷霉素，两种抗生素都能减少粪便中的STEC，然而，用环丙沙星治疗的动物粪便中游离Stx明显增加，与三分之二的小鼠死亡相关，而磷霉素则没有。使用卡那霉素标记的Stx 2原噬菌体的实验表明，环丙沙星，而不是磷霉素，引起Stx 2原噬菌体从一个大肠杆菌肠内转移的增强。大肠杆菌到另一个。这些观察结果表明，用噬菌体诱导抗生素（如氟喹诺酮类）治疗人STEC感染可能具有显著的不良临床后果，并且氟喹诺酮类抗生素可能增强体内毒力因子的运动。
Shiga toxin-producing Escherichia coli (STEC) cause significant disease; treatment is supportive and antibiotic use is controversial. Ciprofloxacin but not fosfomycin causes Shiga toxin-encoding bacteriophage induction and enhanced Shiga toxin (Stx) production from E. coli O157:H7 in vitro. The potential clinical relevance of this was examined in mice colonized with E. coli O157:H7 and given either ciprofloxacin or fosfomycin, Both antibiotics caused a reduction in fecal STEC, However, animals treated with ciprofloxacin had a marked increase in free fecal Stx, associated with death in two-thirds of the mice, whereas fosfomycin did not. Experiments that used a kanamycin-marked Stx2 prophage demonstrated that ciprofloxacin, but not fosfomycin, caused enhanced intraintestinal transfer of Stx2 prophage from one E. coli to another. These observations suggest that treatment of human STEC infection with bacteriophage-inducing antibiotics, such as fluoroquinolones, may have significant adverse clinical consequences and that fluoroquinolone antibiotics may enhance the movement of virulence factors in vivo.