Iodine-125 radiolabeling of silver nanoparticles for in vivo SPECT imaging
Iodine-125 radiolabeling of silver nanoparticles for in vivo SPECT imaging
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DOI：
10.2147/ijn.s11677
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发表时间：
2010-01-01
影响因子：
8
通讯作者：
Schnitzer, Jan E.
中科院分区：
文献类型：
--
作者：
Chrastina, Adrian;Schnitzer, Jan E.
银纳米颗粒越来越多地在医学上得到应用；然而，人们对它们在体内的组织分布知之甚少。在这里，我们开发了一种用碘-125对银纳米粒子进行放射性标记的快速方法，以便通过生物分布分析和单光子发射计算机断层扫描（SPECT）成像来跟踪全身给药后银纳米粒子的体内组织摄取。用碘-125化学吸附法制备了平均尺寸为12 nm的聚（n -乙烯基-2 -吡咯烷酮）包覆银纳米粒子，其放射性标记效率为80%。通过对Balb/c小鼠静脉注射放射性标记的银纳米粒子，通过非侵入性全身SPECT成像评估这些纳米粒子在体内的分布模式，显示纳米粒子在肝脏和脾脏的摄取。生物分布分析证实，银纳米颗粒在24小时内主要积聚在脾脏（41.5% ID/g）和肝脏（24.5% ID/g）。网状内皮系统组织中的广泛摄取表明，进一步研究银纳米颗粒与肝脏和脾脏组织在细胞水平上的相互作用对于评估银纳米颗粒的体内效应和潜在毒性至关重要。这种方法能够快速碘-125放射性标记银纳米颗粒，具有足够的体内成像和生物分布分析的特定活性。
Silver nanoparticles are increasingly finding applications in medicine; however, little is known about their in vivo tissue distribution. Here, we have developed a rapid method for radiolabeling of silver nanoparticles with iodine-125 in order to track in vivo tissue uptake of silver nanoparticles after systemic administration by biodistribution analysis and single-photon emission computerized tomography (SPECT) imaging. Poly(N-vinyl-2 -pyrrolidone)-capped silver nanoparticles with an average size of 12 nm were labeled by chemisorption of iodine-125 with a >80% yield of radiolabeling efficiency. Radiolabeled silver nanoparticles were intravenously injected in Balb/c mice, and the in vivo distribution pattern of these nanoparticles was evaluated by noninvasive whole-body SPECT imaging, which revealed uptake of the nanoparticles in the liver and spleen. Biodistribution analysis confirmed predominant accumulation of the silver nanoparticles in the spleen (41.5% ID/g) and liver (24.5% ID/g) at 24 h. Extensive uptake in the tissues of the reticuloendothelial system suggests that further investigation of silver nanoparticle interaction with hepatic and splenic tissues at the cellular level is critical for evaluation of the in vivo effects and potential toxicity of silver nanoparticles. This method enables rapid iodine-125 radiolabeling of silver nanoparticles with a specific activity sufficient for in vivo imaging and biodistribution analysis.