A whole cell immunization-derived monoclonal antibody that protects cells from coxsackievirus A9 infection binds to both cell surface and virions
A whole cell immunization-derived monoclonal antibody that protects cells from coxsackievirus A9 infection binds to both cell surface and virions
复制标题
DOI：
10.1016/j.jviromet.2005.06.012
复制
发表时间：
2005-12-01
影响因子：
3.1
通讯作者：
Hovi, T
中科院分区：
文献类型：
--
作者：
Roivainen, M;Alakulppi, N;Hovi, T
柯萨奇病毒 A9 (CAV-9) 使用一种未鉴定的 RGD 独立受体感染人横纹肌肉瘤 (RD) 细胞。通过用完整的 RD 细胞免疫小鼠并选择免受 CAV-9 细胞病变效应的细胞进行保护来制备单克隆抗体。在这里，我们描述了一种与宿主细胞质膜结合并保护细胞免受病毒感染的单克隆抗体。此外，在抗体存在的情况下，病毒与细胞单层的结合更有效，这表明该抗体也能够识别病毒颗粒。使用高度纯化的病毒制剂进行的免疫沉淀和电子显微镜研究证实了单克隆抗体与病毒颗粒的结合。该抗体还识别柯萨奇病毒 A21 和脊髓灰质炎病毒的所有三种血清型，但不影响其传染性。通过肽图谱和在抗体存在下产生逃逸突变体来鉴定单克隆抗体识别的CAV-9的氨基酸序列。 (c) 2005 Elsevier B.V. 保留所有权利。
Coxsackievirus A9 (CAV-9) infects human rhabdomyosarcoma (RD) cells using an unidentified RGD-independent receptor. Monoclonal antibodies were prepared by immunizing mice with intact RD cells and by selecting cells from the cytopathic effect of CAV-9 for protection, Here we describe a monoclonal antibody that binds to host cell plasma membrane and protects cells from virus infection. In addition, binding of the virus to cell monolayers was more efficient in the presence of the antibody, suggesting that the antibody is also capable of recognizing virus particles. Immunoprecipitation and electron microscopy studies with highly purified virus preparations verified binding of the monoclonal antibody to the virus particles. The antibody also recognized coxsackievirus A21 and all three serotypes of poliovirus, but without affecting their infectivity. The amino acid sequence of CAV-9 recognized by the monoclonal antibody was identified by peptide mapping and by producing escape mutants in the presence of the antibody. (c) 2005 Elsevier B.V. All rights reserved.