Optimisation of bioimpedance measurements of neuronal activity with an ex vivo preparation of Cancer pagurus peripheral nerves
Optimisation of bioimpedance measurements of neuronal activity with an ex vivo preparation of Cancer pagurus peripheral nerves
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DOI：
10.1016/j.jneumeth.2019.108322
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发表时间：
2019-11-01
影响因子：
3
通讯作者：
Holder, David S.
中科院分区：
文献类型：
--
作者：
Chapman, ChristOpher A. R.;Smith, Trevor M.;Holder, David S.
背景资料：在哺乳动物中，快速神经电阻抗断层扫描（EIT）可以使用神经袖带对复合动作电位（CAP）的有髓鞘成分进行成像。如果应用于无髓纤维，这具有改善选择性神经调节（“电刺激”）以避免脱靶效应的巨大潜力。以前，生物阻抗记录平均从无髓鞘的蟹腿神经纤维，但信噪比（SNR）需要improving.New方法：目前，功能性非侵入性神经元成像是通过表面电极或基因表达的指标，提供良好的空间，但穷人的时间，分辨率完成。这里是一个改进的方法，从模型的无髓纤维的生物阻抗测量，使优化通过改进的1）信号处理测量范式，2）神经生理学，和3）电极-神经interfaces.Results：对于生物阻抗记录，招聘和必要性的CAP进行量化和盐水显着提高了SNR。改进的方案导致不需要平均，因为顺序记录的迹线产生-0.232 +/-0.064%的生物阻抗变化，其不显示相位或定时相关的伪影。与现有方法的比较：这里，两个生物阻抗迹线显示>= 3：1的SNR，而之前需要超过100个平均值，具有更大的实验间可变性。10对痕迹平均SNR>= 9：1，或近实时measurement.Conclusions：这种方法有利于进一步的研究，旨在使非侵入性定位在周围神经内的无髓纤维的分支活动。这项技术最终可以产生第一个三维断层图像，以帮助指导使用生物电设备的选择性神经调节。
Background: In mammals, fast neural Electrical Impedance Tomography (EIT) can image the myelinated component of the compound action potentials (CAP) using a nerve cuff. If applied to unmyelinated fibres this has great potential to improve selective neuromodulation ("electroceuticals") to avoid off-target effects. Previously, bioimpedance recordings were averaged from unmyelinated crab leg nerve fibres, but the signal to noise ratio (SNR) needs improving.New method: Currently, functional non-invasive neuronal imaging is accomplished through surface electrodes or genetically expressed indicators that provide good spatial, but poor temporal, resolution. Here is an improved method for bioimpedance measurements from a model of unmyelinated fibres to enable optimisation through improvement of the 1) signal processing measurement paradigm, 2) neurophysiology, and 3) electrode-nerve interface.Results: For bioimpedance recordings, the recruitment and necessity of the CAP was quantified and saline significantly improved the SNR. An improved protocol resulted in averaging not being required, as sequentially recorded traces produced bioimpedance changes of -0.232 +/- 0.064% that did not show phase or timing related artefacts.Comparison with existing method: Here, two bioimpedance traces displayed an SNR of >= 3:1, while previously over > 100 averages were required with greater inter-experimental variability. 10 paired traces were averaged for an SNR of >= 9:1, or near real-time measurement.Conclusions: This method facilitates further studies aiming to enable non-invasive localization of fascicular activity in unmyelinated fibres within peripheral nerves. This technique could ultimately produce the first 3-D tomographic images to help guide selective neuromodulation using bioelectric devices.