Hematopoietic Stem Cell Protocols
Hematopoietic Stem Cell Protocols
复制标题
造血干细胞方案
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
C. Jordan
中科院分区：
文献类型：
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作者：
C. Klug;C. Jordan
基于细胞周期定位的原始造血细胞的分离与表征小鼠胚胎造血干细胞的分离与分析小鼠成熟顺序阶段造血干细胞的纯化人造血干细胞的流式细胞术纯化方法流式细胞术和人造血干细胞的免疫选择细胞造血集落形成细胞鹅卵石区形成试验多能性骨髓干细胞的试验胸腺内注射用于t细胞祖细胞活性分析的长期培养启动细胞试验人类和小鼠细胞通过限制性稀释分析在竞争性繁殖宿主中定量小鼠和人类造血细胞体外扩增人和小鼠造血干细胞通过逆转录病毒对小鼠造血干细胞进行遗传修饰面部纯化的造血干细胞的逆转录转导逆转录病毒介导的人造血干细胞和祖细胞的转导和克隆整合分析慢病毒载体上清液的制备和细胞靶点的转导逆转录酶-pcr分析hsc中基因表达的分析利用二维基因表达指纹图谱鉴定分选细胞群体中差异表达的基因构建造血干细胞纯化群体cDNA文库培养细胞
Non-invasive measurement of hematopoietic stem cell cycling and turnover by in vivo bromodeoxyuridine incorporation Isolation and characterizationof primitive hematopoietic cells based on their position in cell cycle Isolation and analysis of hematopoietic stem cells from mouse embryos The purification of mouse hematopoietic stem cells at sequential stages of maturation Method for purification of human hematopoietic stem cells by flow cytometry Flow cytometry and immunoselection of human stem cells Hematopoietic colony-forming cells The cobblestone area forming assay CFU-S: an assay for pluripotent myelopoietic stem cells Intrathymic injection for analysis of t cell progenitor activity Long-term culture-initiating cell assays for human and murine cells Quantitation of murine and human hematopoietic cells by limiting-dilution analysis in competitively repopulated hosts Ex vivo expansion of human and murine hematopoietic stem cells Genetic modification of murine hematopoietic stem cells by retroviruses Retroviral transduction of facs-purified hematopoietic stem cells Retroviral-mediated transduction and clonal integration analysis of human hematopoietic stem and progenitor cells Production of lentiviral vector supernatants and transduction of cellular targets Reverse transcriptase-pcr analysis of gene expression in hsc Identification of differentially expressed genes in sorted cell populations by two-dimensional gene expression fingerprinting Construction of cDNA libraries from purified populations of hematopoietic stem cells Hematopoietic development of ES cells in culture