Rapid chromosome identification by oligonucleotide-primed in situ DNA synthesis (PRINS).
Rapid chromosome identification by oligonucleotide-primed in situ DNA synthesis (PRINS).
复制标题
通过寡核苷酸引发的原位 DNA 合成 (PRINS) 快速鉴定染色体。
作者：
J. Gosden;D. Lawson
我们描述了一种方法，用于快速鉴定染色体在中期，和定量的染色体在间期，退火寡核苷酸引物，来自染色体特异性子集的重复DNA家族，细胞学制剂的DNA，和酶促延伸与掺入标记的核苷酸。该方法同样适用于正常细胞或来自体细胞杂种的细胞。当标记的产物太小或拷贝数太低而在单次延伸反应后不容易看到时，我们开发了一种用于标记的DNA的循环扩增的方法，使得能够清楚地可视化信号。
We describe a method for rapid identification of chromosomes at metaphase, and quantification of chromosomes in interphase, by annealing oligonucleotide primers, derived from chromosome-specific subsets of repeated DNA families, to the DNA of cytological preparations, and enzymatic extension with the incorporation of labelled nucleotides. The method is equally applicable to normal cells or those from somatic cell hybrids. Where the labelled product is too small or of too low a copy number to be readily seen after the single extension reaction, we have developed a method for cyclic amplification of the labelled DNA, enabling clear visualization of the signal.