Down-Regulation of Phosphoribosyl Pyrophosphate Synthetase 1 Inhibits Neuroblastoma Cell Proliferation
Down-Regulation of Phosphoribosyl Pyrophosphate Synthetase 1 Inhibits Neuroblastoma Cell Proliferation
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DOI：
10.3390/cells8090955
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发表时间：
2019-09-01
期刊：
影响因子：
6
通讯作者：
Cui, Hongjuan
中科院分区：
文献类型：
--
作者：
Li, Jifu;Ye, Junhong;Cui, Hongjuan
磷酸核糖焦磷酸合成酶1（PRPS 1）是核苷酸从头合成和核苷酸补救合成途径中的关键酶，这些途径对嘌呤和嘧啶生物合成至关重要。PRPS1的异常高表达除了与神经母细胞瘤相关外，还可引起许多疾病，包括听力损失、张力减退和共济失调。然而，PRPS1在神经母细胞瘤中的作用仍不清楚。在这项研究中，我们发现PRPS1通常在神经母细胞瘤细胞中表达，并且与癌症的不良预后密切相关。此外，PRPS1的下调通过干扰DNA合成在体外和体内抑制神经母细胞瘤细胞增殖和肿瘤生长。这项研究为神经母细胞瘤患者的治疗和药物开发提供了新的见解。
Phosphoribosyl pyrophosphate synthetase 1 (PRPS1) is a key enzyme in de novo nucleotide synthesis and nucleotide salvage synthesis pathways that are critical for purine and pyrimidine biosynthesis. Abnormally high expression of PRPS1 can cause many diseases, including hearing loss, hypotonia, and ataxia, in addition to being associated with neuroblastoma. However, the role of PRPS1 in neuroblastoma is still unclear. In this study, we found that PRPS1 was commonly expressed in neuroblastoma cells and was closely related to poor prognosis for cancer. Furthermore, down-regulation of PRPS1 inhibited neuroblastoma cell proliferation and tumor growth in vitro and in vivo via disturbing DNA synthesis. This study provides new insights into the treatment of neuroblastoma patients and new targets for drug development.