Spatiotemporal expression of connexin39 and-43 during myoblast differentiation in cultured cells and in the mouse embryo
Spatiotemporal expression of connexin39 and-43 during myoblast differentiation in cultured cells and in the mouse embryo
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DOI：
10.1080/15419060600631508
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发表时间：
2006-01-01
影响因子：
--
通讯作者：
Willecke, K
中科院分区：
文献类型：
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作者：
von Maltzahn, J;Wulf, V;Willecke, K
已知连接蛋白39（Cx39）和连接蛋白43（Cx43）在骨骼肌发育过程中表达。在这里，我们比较了两个连接蛋白的表达模式，在分化过程中建立的C2C12小鼠成肌细胞和小鼠胚胎。Cx43在未分化的成肌细胞中高度丰富，但在这些细胞中未检测到Cx39蛋白。在分化成肌管后，Cx39表达增加。在小鼠胚胎中也观察到这些连接蛋白的连续表达。Cx39和Cx43在不同的斑块中被发现，这与Cx43只在成肌细胞中表达而Cx39只在肌管中表达的观点一致。因此，在培养中分化C2C12细胞可以用于研究肌发生中间隙连接的参与，因为相应的Cx39和Cx43蛋白的表达似乎与小鼠胚胎中非常相似。
Connexin39 (Cx39) and connexin43 (Cx43) are known to be expressed during development of skeletal muscles. Here we have compared the expression pattern of both connexins during differentiation of established C2C12 mouse myoblasts and in the mouse embryo. Cx43 is highly abundant in undifferentiated myoblasts, but no Cx39 protein was detected in these cells. Upon differentiation into myotubes, Cx39 expression increased. The consecutive expression of these connexins was also observed in the mouse embryo. Cx39 and Cx43 were found in different plaques in accordance with the notion that Cx43 is exclusively expressed inmyoblasts and Cx39 in myotubes. Thus, differentiating C2C12 cells in culture can serve to study the involvement of gap junctions in myogenesis, since expression of corresponding Cx39 and Cx43 proteins appears to be very similar as in the mouse embryo.