Development of quantitative NMR method with internal standard for the standard solutions of paralytic shellfish toxins and characterisation of gonyautoxin-5 and gonyautoxin-6
Development of quantitative NMR method with internal standard for the standard solutions of paralytic shellfish toxins and characterisation of gonyautoxin-5 and gonyautoxin-6
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DOI：
10.1016/j.toxicon.2010.06.002
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发表时间：
2010-09-15
期刊：
影响因子：
2.8
通讯作者：
Oshima, Yasukatsu
中科院分区：
文献类型：
--
作者：
Watanabe, Ryuichi;Suzuki, Toshiyuki;Oshima, Yasukatsu
The chemical analysis of paralytic shellfish toxins (PSTs) requires standard solutions with accurate concentration. The mouse toxicity in each toxin is also essential knowledge for the introduction of chemical analysis as an alternative method to mouse bioassay (MBA) in routine monitoring of shellfish In this study, we developed the quantitative analysis of PSTs by nuclear magnetic resonance (NMR), using tert-butanol as an internal standard. Only proton signals with longitudinal relaxation time (T-1) of less than 2.5 s, including the internal standard, were used for quantitation of toxins. Our method showed good precision (
