Influence of red cell water content on the morphology of sickling.
Influence of red cell water content on the morphology of sickling.
复制标题
红细胞含水量对镰状细胞形态的影响。
作者：
M. Clark;J. Guatelli;N. Mohandas;S. Shohet
已经研究了不同含水量的镰状细胞对氧张力变化的反应。即使在长时间复氧后，镰状细胞严重脱水仍保留特征性的镰状形态。当通过降低悬浮介质渗透压而增加细胞水含量时，细胞脱镰。脱氧前脱水的细胞无法呈现患病细胞典型的针状形态。对于高平均细胞血红蛋白浓度 (MCHC) 盘状镰状细胞和不可逆镰状细胞来说都是如此。当这些细胞在脱氧前重悬于低渗培养基中时，它们会形成镰状细胞，具有正常 MCHC 镰状细胞的特征形态。负载 Ca 的镰状细胞以及不可逆镰状细胞的形态行为显示出血红蛋白浓度增加和极高的内部粘度的主要影响。没有观察到假定的膜刚性对细胞形态的限制。
The response of sickle cells with varying water content to alterations in oxygen tension has been studied. Cells that were severely dehydrated while sickled retained the characteristic sickled morphology even after prolonged reoxygenation. When the cell water content was increased by reduction of the suspending medium osmolality, the cells unsickled. Cells that were dehydrated before deoxygenation were unable to assume the spiculated morphology typical of sicked cells. This was true both for high mean cell hemoglobin concentration (MCHC) discoid sickle cells and for irreversibly sickled cells. When such cells were resuspended in hypotonic medium before deoxygenation, they sickled with the characteristic morphology of sickle cells with normal MCHC. The morphological behavior of Ca-loaded sickled cells as well as irreversibly sickled cells showed a major influence of increased hemoglobin concentration and extremely high internal viscosity. Constraint on cell morphology by putative membrane rigidity was not observed.