Effect of heat stress on peroxidase activity and total protein content in strawberry plants
Effect of heat stress on peroxidase activity and total protein content in strawberry plants
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DOI：
10.1016/j.plantsci.2003.11.014
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发表时间：
2004-03-01
期刊：
影响因子：
5.2
通讯作者：
Eris, A
中科院分区：
文献类型：
--
作者：
Gulen, H;Eris, A
研究了高温对草莓(Fragaria x Ananassa cv.)过氧化物酶(PRX)同工酶活性和叶片蛋白质的影响。卡马罗萨)。苗木用珍珠岩培养3周，昼夜温度25/10℃，每天用1/3强度的改良Hoagland营养液浇水。一半的植物被转移到恒定25摄氏度的生长室中一周，以适应植物。将温度逐步升高(5摄氏度/48小时)至30、35、40摄氏度，最后升至45摄氏度。除了驯化的植物，新的植物从外部转移到生长室，在每个温度步骤施加热击。总体而言，渐进式热应激(GHS)和冲击性热应激(SHS)对研究变量的影响是显著的。对高温处理的反应，所有样品的PRX活性都很高。相反，热胁迫降低了总蛋白质含量。与SHS植物的叶片相比，GHS植物对高温的反应表现出更高的PRX酶活性。在聚丙烯酰胺凝胶电泳法(PAGE)中，所有不同强度的样品均检测到一条基本的Prx条带(Rf=0.22)。此外，与暴露在SHS中的植物相比，暴露在GHS中的植物从叶片中泄漏的电解质更少。(C)2003爱思唯尔爱尔兰有限公司。保留所有权利。
Effects of high temperature on the activity of peroxidase (PRX) isozyme and leaf proteins were studied in strawberry (Fragaria x ananassa cv. Camarosa). Seedlings were grown using perlite for 3 weeks at 25/10 degreesC day/night temperature, and watered daily by 1/3 strength modified Hoagland nutrient solution. Half of the plants were transferred to a growth chamber with a constant 25 degreesC for a week to acclimate the plants. Temperature was increased stepwise (5 degreesC/48 h) to 30, 35, 40 degreesC and finally to 45 degreesC. In addition to acclimated plants, new plants were transferred from outside to the growth chamber, at each temperature step to impose a heat shock. In general, effects of gradual heat stress (GHS) and shock heat stress (SHS) on the variables studied were significant. PRX activities were high in all the samples in response to high temperature treatment. Conversely, total protein content was decreased by heat stress. GHS plants showed significantly higher activities of PRX enzyme in response to high temperature compared to those from the leaves of SHS plants. One basic PRX band (rf = 0.22) was detected in all the samples with different intensity in polyacrylamide gel electrophoresis (PAGE). In addition, plants exposed to GHS leaked less electrolytes from the leaves compared with the plants exposed to SHS. (C) 2003 Elsevier Ireland Ltd. All rights reserved.