Antihypertensive Effect of Enzymatic Hydrolysate of Collagen and Gly-Pro in Spontaneously Hypertensive Rats
Antihypertensive Effect of Enzymatic Hydrolysate of Collagen and Gly-Pro in Spontaneously Hypertensive Rats
复制标题
DOI：
10.1271/bbb.90197
复制
发表时间：
2009-10-01
影响因子：
1.6
通讯作者：
Maruyama, Susumu
中科院分区：
文献类型：
--
作者：
Ichimura, Toshiaki;Yamanaka, Akiko;Maruyama, Susumu
连续口服猪皮胶原蛋白酶解物对自发性高血压大鼠（SHR）有降压作用。我们从水解物中分离出一种血管紧张素I转化酶（ACE）抑制肽Gly-Phe-Hyp-Gly-Pro（IC_（50）= 91 μ M），但其他分离的肽的ACE抑制活性较弱。虽然Gly-Pro的ACE抑制活性（IC 50 = 360 μ M）不强，但Gly-Pro作为大量重复序列存在于胶原中。然后，我们检查了Gly-Pro的抗高血压作用。口服500 mg/kg的Gly-Pro显著降低SHR的血压，并且在50 mg/kg时也显示出降低血压的趋势。口服Gly-Phe-Hyp-Gly-Pro（10或30 mg/kg）也可降低SHR的血压。
Continuous oral feeding of enzymatic hydrolysate of porcine skin collagen showed an anti hypertensive effect in spontaneously hypertensive rats (SHRs). We isolated an angiotensin I-converting enzyme (ACE) inhibitory peptide, Gly-Phe-Hyp-Gly-Pro (IC50 = 91 mu M), from the hydrolysate, but the ACE inhibitory activities of the other peptides isolated were weak. Although the ACE inhibitory activity of Gly-Pro (IC50 = 360 mu M) was not potent, Gly-Pro exists in collagen as a large number of repeated sequences. We then examined the antihypertensive effect of Gly-Pro. Orally administered Gly-Pro at 500 mg/kg significantly decreased the blood pressure of SHRs, and at 50 mg/kg it also showed a tendency to lower the blood pressure. Oral administration of Gly-Phe-Hyp-Gly-Pro (10 or 30 mg/kg) also decreased the blood pressure of SHRs.