The high-affinity phosphodiesterase PcPdeH is involved in the polarized growth and pathogenicity of Phytophthora capsici
The high-affinity phosphodiesterase PcPdeH is involved in the polarized growth and pathogenicity of Phytophthora capsici
复制标题
高亲和力磷酸二酯酶 PcPdeH 参与辣椒疫霉的极化生长和致病性
DOI：
10.1016/j.funbio.2020.01.006
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发表时间：
2020-03-01
期刊：
影响因子：
2.5
通讯作者：
Zhang, Deyong
中科院分区：
文献类型：
--
作者：
Li, X.;Liu, Yong;Zhang, Deyong
已证明cAMP信号通路在控制植物病原体的形态变化和致病性方面非常重要。在本研究中，我们确定了PcPdeH，一个基因编码的高亲和力磷酸二酯酶（PDE），这是一个关键的调节cAMP信号通路。为了阐明PcPdeH的功能，使用II型CRISPR/Cas9系统在辣椒疫霉中获得PcPdeH敲除突变体。PcPdeH基因敲除转化子表现出营养生长缺陷和异常孢囊萌发。感染试验表明，与野生型相比，PcPdeH基因敲除突变体对辣椒和烟草叶片的毒力显著降低，cAMP水平相对于野生型和CK菌株增加了1.5-2倍。基于这些表型特征，我们认为PcPdeH对辣椒疫霉的营养生长、孢囊萌发和致病性至关重要。(C)2020作者（S）由Elsevier Ltd代表英国真菌学会出版。
The cAMP signaling pathway has been shown to be important in controlling morphological changes and pathogenicity in plant pathogens. In the present study, we identified PcPdeH, a gene encoding a high-affinity phosphodiesterase (PDE), which is a key regulator of the cAMP signaling pathway. To elucidate the function of PcPdeH, PcPdeH-knockout mutants were obtained using a type II CRISPR/Cas9 system in Phytophthora capsici. The knockout transformants of PcPdeH showed vegetative growth defects and abnormal cyst germination. Infection assays indicated that compared with the wild type, PcPdeH-knockout mutants showed significantly reduced virulence on pepper and tobacco leaves and exhibited increased (1.5-2-fold) cAMP levels relative to the wild-type and CK strains. Based on these phenotypic features, we propose that PcPdeH is crucial for vegetative growth, cyst germination and pathogenicity in P. capsici. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of British Mycological Society.