Comparative transcriptome analysis of transcription factors in different maize varieties under salt stress conditions
Comparative transcriptome analysis of transcription factors in different maize varieties under salt stress conditions
复制标题
盐胁迫条件下不同玉米品种转录因子的比较转录组分析
DOI：
10.1007/s10725-016-0192-9
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发表时间：
2017-01-01
影响因子：
4.2
通讯作者：
Jin, Chao
中科院分区：
文献类型：
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作者：
Du, Xilong;Wang, Gang;Jin, Chao
盐胁迫是影响植物生长和作物产量的主要环境因子，转录因子在植物对盐胁迫的响应中起着至关重要的作用。识别与耐盐相关的基因有助于耐盐作物育种。采用比较转录组分析方法，研究了两个不同耐盐性玉米品种的全长转录因子的整体基因表达。共鉴定出55个Tf家系，1283个Tf基因。其中，314个Tf基因在盐胁迫下在两个玉米品种中差异表达。检测到177个TF基因在耐盐品种中的表达水平显著高于盐敏感品种。一组转录因子家族的差异表达清楚地表明了它们在耐盐性中的重要作用。进一步的系统发育分析和基因表达分析表明，大多数热激因子都是由盐胁迫诱导的，但不同的类/亚类对盐胁迫的反应不同。在盐胁迫下，耐盐品种HSF-B类基因的表达水平显著高于盐敏感品种，这可能导致了不同植物的耐盐能力不同。这些结果有助于更好地理解玉米转录因子响应盐胁迫的复杂机制，并为进一步研究转录因子家族的系统分析和揭示其在植物耐盐性中的潜在功能提供了新的视角。
Salt stress is a major environmental factor affecting plant growth and crop production worldwide, and the transcription factors (TFs) play crucial roles in plant response to salt stress. Identifying genes related to salt-tolerance contributes to salt-tolerant crop breeding. A comparative transcriptome analysis was carried out to investigate global gene expression of the entire TFs in two maize varieties with different salt-tolerant ability. Fifty-five TF families including 1283 TF genes were identified. Among them, 314 TF genes were differentially expressed in the two maize varieties under salt stress. 177 TF genes were detected with significantly higher expression levels in salt-tolerance variety compared with the salt-sensitive one. The differential expression of a set of TF families clearly demonstrated their important roles in salt tolerance. Further phylogenetic analysis and gene expression analysis of heat shock factors (HSFs) revealed that majority of these TFs were induced by salt stress, but different classes/subclasses had different response to salt stress. HSF class-B genes were detected with significantly higher expression levels in salt-tolerance variety compared with the salt-sensitive one under salt stress, which may result in different plants salt-tolerance ability. These results contribute to a better understanding of the complex mechanism of TFs in response to salt stress in maize and provide new sight for further research to perform systematic analysis of the TF families and to reveal their potential functions in the salt-tolerance for plants.