Label-free electrochemical immunosensor for sensitive detection of kanamycin
Label-free electrochemical immunosensor for sensitive detection of kanamycin
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DOI：
10.1016/j.snb.2011.01.019
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发表时间：
2011-07
影响因子：
8.4
通讯作者：
Yanfang Zhao;Q. Wei;Caixia Xu;He Li;Dan Wu;Yan-yan Cai;Kexia Mao;Z. Cui;B. Du
中科院分区：
文献类型：
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作者：
Yanfang Zhao;Q. Wei;Caixia Xu;He Li;Dan Wu;Yan-yan Cai;Kexia Mao;Z. Cui;B. Du
报道了一种基于水溶性石墨烯片（WGS）/普鲁士蓝壳聚糖（PB-CTS）/纳米多孔金（NPG）复合膜的新型卡那霉素免标记电化学免疫传感器。以PB为电子传递介质，通过静电吸附将其与WGS一起修饰在电极上。然后将NPG固定到所制备的膜上用于生物分子的锚定。WGS和NPG的存在大大提高了PB的电活性。这主要是由于WGS和NPG良好的导电性促进了电子转移，提高了灵敏度。以卡那霉素抗体为模型，将卡那霉素抗体固定在复合膜上，用于检测卡那霉素。在最佳条件下，卡那霉素浓度在0.02- 14 ngmL-1范围内与PB的安培信号呈线性关系，线性关系为y=1.3817+4.7544x，r=0.9993，检出限为6.31pgmL-1。该传感器用于真实的样品中卡那霉素的检测，结果令人满意。该方法也适用于动物源性食品中其他抗生素残留的检测。
A novel label-free electrochemical immunosensor for sensitive detection of kanamycin based on water-soluble graphene sheet (WGS)/prussian blue-chitosan (PB-CTS)/nanoporous gold (NPG) composited film has been reported. PB was selected as an electron transfer mediator, and was modified onto the electrode together with WGS through electrostatic adsorption. Then NPG was immobilized onto the as-prepared film for biomolecules anchoring. The electroactivity of PB was greatly enhanced in the presence of WGS and NPG. It could mainly be ascribed to the fact that the good conductivity of WGS and NPG promoted electron transfer and enhanced the sensitivity. kanamycin antibody, as a model, was immobilized onto the composite film for the detection of kanamycin. Under optimum conditions, the amperometric signal of PB decreased linearly with kanamycin concentration (0.02–14ngmL−1), a linear calibration plot (y=1.3817+4.7544x, r=0.9993), resulting in a low limit of detection (6.31pgmL−1). The novel immunosensor for the detection of kanamycin in real sample with satisfactory results has been proved. In addition, this method would be easily adapted for the detection of other residual antibiotics in animal derived foods.