Nitrogen dynamics, bacterial community composition and biofloc quality in biofloc-based systems cultured Oreochromis niloticus with poly-β-hydroxybutyric and polycaprolactone as external carbohydrates
Nitrogen dynamics, bacterial community composition and biofloc quality in biofloc-based systems cultured Oreochromis niloticus with poly-β-hydroxybutyric and polycaprolactone as external carbohydrates
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DOI：
10.1016/j.aquaculture.2017.07.017
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发表时间：
2017-10
期刊：
影响因子：
4.5
通讯作者：
Guozhi Luo;N. Zhang;Shengli Cai;Hongxin Tan;Zefeng Liu
中科院分区：
文献类型：
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作者：
Guozhi Luo;N. Zhang;Shengli Cai;Hongxin Tan;Zefeng Liu
控制C：N比例是减少水产养殖系统中铵态氮（NH 4 +-N）积累的一种实用方法。本研究以聚-β-羟基丁酸（PHB）和聚己内酯（PCL）为碳源，研究了罗非鱼养殖系统的碳源组成。120天没有换水。在PHB组（37.93 ± 8.87 kg m− 3）和PCL组（34.29 ± 9.29 kg m− 3）之间，或者在PHB组和葡萄糖作为外部碳水化合物的组（44.14 ± 10.51 kg m− 3）之间，没有观察到最终生物量的显著差异。在稳定状态下，NH 4 +-N和亚硝酸盐氮（NO2−-N）分别< 3.0 mg L− 1和0.8 mg L− 1。在从鱼缸中取出的生物絮体的批处理试验中观察到NH 4 +-N和硝酸盐氮（NO3−-N）的同化、硝化和反硝化。生物絮体的粗蛋白含量（31-39%）在PCL组和PHB组之间以及在PHB组和葡萄糖组之间没有检测到显著差异。通过高通量测序评估的微生物分析显示，三组之间的细菌群落相似。虽然不是每个参数都优于葡萄糖，但由于更方便，仍然认为PHB或PCL优于葡萄糖。总的来说，我们的研究结果表明，聚羟基丁酸酯和PCL是有利的和方便的外部碳水化合物的生物絮体为基础的罗非鱼养殖系统。
Manipulating the C:N ratio is a practical way to reduce the accumulation of ammonium nitrogen (NH4+-N) in an aquaculture system. In this study, we investigated the use of poly-β-hydroxybutyric (PHB) and polycaprolactone (PCL) as carbon sources to support a limited discharge tilapia aquaculture system conveniently. The water was not changed for 120 days. No significant differences in the final biomass were observed between the PHB group (37.93 ± 8.87 kg m− 3) and the PCL group (34.29 ± 9.29 kg m− 3) or between the PHB group and the group with glucose as an external carbohydrate (44.14 ± 10.51 kg m− 3). NH4+-N and nitrite nitrogen (NO2−-N) were < 3.0 mg L− 1and 0.8 mg L− 1at steady state, respectively. The assimilation of NH4+-N and nitrate nitrogen (NO3−-N), nitrification and denitrification were observed in the batch tests of the bioflocs taken from the fish tank. No significant differences were detected in the bioflocs' crude protein content (31–39%) between the PCL group and the PHB group or between the PHB group and the glucose group. Microbial analysis, assessed by high-throughput sequencing, revealed that the bacterial community was similar between the three groups. Although not every parameter was better than glucose, PHB or PCL was still considered preferable to glucose because of greater convenience. Collectively, our results showed that both PHB and PCL are favorable and convenient external carbohydrates for biofloc-based tilapia aquaculture systems.