PROTEIN S-ACYL TRANSFERASE10 Is Critical for Development and Salt Tolerance in Arabidopsis[W]
PROTEIN S-ACYL TRANSFERASE10 Is Critical for Development and Salt Tolerance in Arabidopsis[W]
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DOI：
10.1105/tpc.112.108829
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发表时间：
2013-03
期刊：
影响因子：
11.6
通讯作者：
Liang-Zi Zhou;Sha Li;Qiang-Nan Feng;Yu-ling Zhang;Xin-Ying Zhao;Yonglun Zeng;Hao Wang;Liwen Jiang;Yan Zhang
中科院分区：
文献类型：
--
作者：
Liang-Zi Zhou;Sha Li;Qiang-Nan Feng;Yu-ling Zhang;Xin-Ying Zhao;Yonglun Zeng;Hao Wang;Liwen Jiang;Yan Zhang
S-acylation or palmitoylation, catalyzed by protein S-acyl transferases (PATs), regulates protein targeting and function. This work shows that Arabidopsis PAT10 regulates pleiotropic developmental processes and stress responses. Putative PAT10 substrates include several calcineurin B-like proteins, whose tonoplast association depends on the function of PAT10. Protein S-acylation, commonly known as palmitoylation, is a reversible posttranslational modification that catalyzes the addition of a saturated lipid group, often palmitate, to the sulfhydryl group of a Cys. Palmitoylation regulates enzyme activity, protein stability, subcellular localization, and intracellular sorting. Many plant proteins are palmitoylated. However, little is known about protein S-acyl transferases (PATs), which catalyze palmitoylation. Here, we report that the tonoplast-localized PAT10 is critical for development and salt tolerance in Arabidopsis thaliana. PAT10 loss of function resulted in pleiotropic growth defects, including smaller leaves, dwarfism, and sterility. In addition, pat10 mutants are hypersensitive to salt stresses. We further show that PAT10 regulates the tonoplast localization of several calcineurin B–like proteins (CBLs), including CBL2, CBL3, and CBL6, whose membrane association also depends on palmitoylation. Introducing a C192S mutation within the highly conserved catalytic motif of PAT10 failed to complement pat10 mutants, indicating that PAT10 functions through protein palmitoylation. We propose that PAT10-mediated palmitoylation is critical for vacuolar function by regulating membrane association or the activities of tonoplast proteins.
