Identification of two distinct bovine parainfluenza virus type 3 genotypes
Identification of two distinct bovine parainfluenza virus type 3 genotypes
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DOI：
10.1099/vir.0.2008/000026-0
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发表时间：
2008-07-01
影响因子：
3.8
通讯作者：
Mahony, Timothy John
中科院分区：
文献类型：
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作者：
Horwood, Paul Francis;Gravel, Jennifer Lillian;Mahony, Timothy John
本研究完成了7株牛副流感病毒3型（BPIV-3）临床分离株基质（M）蛋白的部分基因测序和1株代表性分离株（Q5592）的完全测序。由于内部BPIV-3 RT-PCR方法未能产生4个分离株的预期产物，因此启动了核苷酸序列分析。基于M蛋白和整个基因组的核苷酸序列，使用所有可用的BPIV-3核苷酸序列进行系统发育重建，证明存在两种不同的BPIV-3基因型（BPIV-3a和BPIV-3b）。这些新鉴定的基因型对BPIV-3分子检测方法的发展具有意义，并且也可能影响BPIV-3疫苗制剂。
The partial gene sequencing of the matrix (M) protein from seven clinical isolates of bovine parainfluenza virus type 3 (BPIV-3), and the complete sequencing of a representative isolate (Q5592) was completed in this study. Nucleotide sequence analysis was initiated because of the failure of in-house BPIV-3 RT-PCR methods to yield expected products for four of the isolates. Phylogenetic reconstructions based on the nucleotide sequences for the M-protein and the entire genome, using all of the available BPIV-3 nucleotide sequences, demonstrated that there were two distinct BPIV-3 genotypes (BPIV-3a and BPIV-3b). These newly identified genotypes have implications for the development of BPIV-3 molecular detection methods and may also impact on BPIV-3 vaccine formulations.