MORPHOGENESIS AND METABOLISM OF AMPHIBIAN LARVAE AFTER EXCISION OF HEART .2. MORPHOGENESIS OF HEARTLESS LARVAE OF AMBLYSTOMA PUNCTATUM
MORPHOGENESIS AND METABOLISM OF AMPHIBIAN LARVAE AFTER EXCISION OF HEART .2. MORPHOGENESIS OF HEARTLESS LARVAE OF AMBLYSTOMA PUNCTATUM
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DOI：
10.1002/ar.1091180406
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发表时间：
1954-01-01
影响因子：
--
通讯作者：
QUINN, BL
中科院分区：
文献类型：
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作者：
KEMP, NE;QUINN, BL
Harrison期40、41或42期摘除心脏后形成无循环的点状芽囊线虫幼虫存活，并进行有限分化，最长可达15天。心脏切除术后，鳃大小退化，四肢仅有轻微发育。眼睛仍然很小，不能动弹，玻璃体破裂。头部前部生长减少导致小头畸形。黑色素细胞以不同的速度收缩，但最终无情的动物变成了“白化病”。在肠道的前肾小管、隆突后窦、背主动脉、背侧肠系膜和中胚层鞘，以及胃十二指肠区域的体腔内，液体膨胀。消化道继续分化，但分化速度慢于正常。肠道上皮细胞对卵黄小板层的消化明显迟缓。心脏切除后，脾不再发育。无心动物四肢、头骨和椎体原基的软骨分化，仅有少量生长。这些结果强调了循环血液对于调节静水压力和转移持续分化和生长所必需的代谢物的重要性。
Larvae of Amblystoma punctatum rendered circulationless by excision of heart at Harrison stage 40, 41 or 42 survived and underwent limited differentiation for periods up to 15 days. Gills regressed in size and limbs developed only slightly after cardiectomy. Eyes remained small and immovable and suffered collapse of the vitreous chamber. Reduced growth of the anterior part of the head resulted in microcephaly. Melanophores became contracted at variable rates, but eventually heartless animals became "albino. " Internally the pronephric tubules, postcardinal sinuses, dorsal aorta, dorsal mesentery and mesodermal sheath of the gut, and the coelom in the gastroduodenal region became distended with fluid. The digestive tract continued to differentiate but at a rate slower than normal. Digestion of yolk platelets in the intestinal epithelium was considerably retarded. The spleen developed no further after cardiectomy. Cartilages of the limb, skull and vertebral primordia of heartless animals differentiated and grew only slightly. These results underline the importance of circulating blood for regulation of hydrostatic pressure and for the transfer of metabolites necessary for continuing differentiation and growth.