Ozone-induced acute pulmonary fibrosis in rats. Prevention of increased rates of collagen synthesis by methylprednisolone.
Ozone-induced acute pulmonary fibrosis in rats. Prevention of increased rates of collagen synthesis by methylprednisolone.
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臭氧诱导大鼠急性肺纤维化。
DOI：
10.1164/arrd.1981.123.1.47
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
J. Last
中科院分区：
文献类型：
--
作者：
T. Hesterberg;J. Last
暴露于 0.8 至 1.5 ppm（1.6 至 3.0 mg/m3）臭氧 7 天的大鼠制备的肺碎屑的胶原蛋白合成净速率以剂量依赖性方式增加，比仅呼吸过滤空气的大鼠制备的肺碎屑获得的净合成速率高几倍。在暴露于臭氧期间同时施用甲基强的松龙（1至50毫克/公斤/天）可以以剂量依赖的方式阻止胶原蛋白合成速率的增加，对于所测试的每个臭氧水平而言。这些结果可能与在相同类固醇治疗的大鼠中测量到的较低水平的炎性水肿相关，因为其右心尖肺叶湿重降低。我们的结论是，根据损伤的严重程度，通过同时给予高水平的抗炎类固醇甲基强的松龙，可以部分或完全预防由大鼠暴露于高水平的吸入臭氧引起的急性肺纤维化。
The net rate of collagen synthesis by lung minces prepared from rats exposed for 7 days to 0.8 to 1.5 ppm (1.6 to 3.0 mg/m3) of ozone was increased in a dose-dependent manner severalfold above the net rate obtained with lung minces prepared from rats that had breathed only filtered air. Concurrent administration of methylprednisolone (1 to 50 mg/kg/day) during the exposure to ozone prevented the increase in rate of collagen synthesis in a dose-dependent manner for each level of ozone tested. These results could be correlated with lower levels of inflammatory edema measured in the same steroid-treated rats as decreased wet weights of their right apical lung lobes. We conclude that acute pulmonary fibrosis caused by exposure of rats to high levels of inspired ozone can be partially or completely prevented, depending on the severity of the insult, by simultaneous administration of high levels of the anti-inflammatory steroid methylprednisolone.