Terminating DNA Tile Assembly with Nanostructured Caps
Terminating DNA Tile Assembly with Nanostructured Caps
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DOI：
10.1021/acsnano.7b02256
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发表时间：
2017-10-01
期刊：
影响因子：
17.1
通讯作者：
Schulman, Rebecca
中科院分区：
文献类型：
--
作者：
Agrawal, Deepak K.;Jiang, Ruoyu;Schulman, Rebecca
对自组装过程的成核、生长和终止的精确控制是控制产品产量和组装动力学的基本工具。以编程方式改变这些过程的机制可以允许使用简单的组件来自组装复杂的最终产品或设计允许动态组装或重新配置的过程。在这里，我们使用DNA瓦片自组装开发一般的设计原则，用于构建复合物，可以结合到一个不断增长的生物分子组装，并终止其增长，通过系统地表征不同的DNA折纸纳米结构如何与DNA瓦片纳米管的增长端相互作用。我们发现，纳米结构，目前的结合界面上的所有结合位点的生长面可以选择性地结合到生长的两端，并停止生长时，这些接口是一个刚性或松软的支架。相比之下，纳米管的成核需要结合位点以与结构的刻面形状相匹配的排列呈现。因此，有可能构建可以终止现有纳米管生长但不能使新结构成核的纳米结构。所得到的用于构建引导一维纳米结构的生长的成核和终止的结构的设计原理也可以用作使用纳米结构设计以编程方式引导二维和三维结晶过程的起点。
Precise control over the nucleation, growth, and termination of self-assembly processes is a fundamental tool for controlling product yield and assembly dynamics. Mechanisms for altering these processes programmatically could allow the use of simple components to self-assemble complex final products or to design processes allowing for dynamic assembly or reconfiguration. Here we use DNA tile self-assembly to develop general design principles for building complexes that can bind to a growing biomolecular assembly and terminate its growth by systematically characterizing how different DNA origami nanostructures interact with the growing ends of DNA tile nanotubes. We find that nanostructures that present binding interfaces for all of the binding sites on a growing facet can bind selectively to growing ends and stop growth when these interfaces are presented on either a rigid or floppy scaffold. In contrast, nucleation of nanotubes requires the presentation of binding sites in an arrangement that matches the shape of the structure's facet. As a result, it is possible to build nanostructures that can terminate the growth of existing nanotubes but cannot nucleate a new structure. The resulting design principles for constructing structures that direct nucleation and termination of the growth of one-dimensional nanostructures can also serve as a starting point for programmatically directing two- and three-dimensional crystallization processes using nanostructure design.