Nanoformulation approach for improved stability and efficiency of lactoperoxidase
Nanoformulation approach for improved stability and efficiency of lactoperoxidase
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DOI：
10.1080/10826068.2020.1848866
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发表时间：
2020-11-12
影响因子：
2.9
通讯作者：
El-Fakharany, Esmail M.
中科院分区：
文献类型：
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作者：
El-Fakharany, Esmail M.
乳过氧化物酶是一种糖基化蛋白质，分子量为78 kDa，在多种哺乳动物的分泌物中分泌。乳过氧化物酶被包括在许多生物过程中，并且众所周知具有杀生物作用，作为活性抗生素和抗病毒剂出席。这种广谱的杀生物活性通过称为乳过氧化物酶系统的明确抑制系统介导，该系统在先天免疫应答中起有效作用，因为其活性不受抗微生物作用的限制，但可能在许多毒素如黄曲霉毒素的水解中起重要作用。因此，随着当前技术的进步，纳米颗粒可以提供机会作为活性候选物，其可以用于稳定和增强LPO的活性以用于多种应用中。由于LPO的可变性功能，这种酶认为活性靶标被封装或涂覆到NP上，用于开发具有受控表面特征的新型纳米组合。通过纳米制剂可以提高LPO构象稳定性数周的方法的开发可以提高这种生物活性成分的生物制药适用性。LPO的纳米制剂增强了新的功能，这些功能可用于许多生物技术应用，如食品工业、化妆品和制药应用，或用于递送和包封生物活性组分。
Lactoperoxidase is a glycosylated protein with a molecular mass of 78 kDa, which being excreted in several mammalian secretions. Lactoperoxidase is included in many biological processes and well-known to have biocidal actions, attending as active antibiotics and antiviral agents. This wide-spectrum of biocidal activities mediates via a definite inhibitory system named lactoperoxidase system which acts a potent role in the innate immune response since its activity is not restricted by the antimicrobial effect, but might act a significant role in the hydrolysis of many toxins like aflatoxin. Hence with the current progresses in technology, nanoparticles can offer chances as an active candidate that might be utilized for stabilizing and potentiating the activity of LPO for use in several applications. Due to the variability functions of LPO, this enzyme considers an active target to be encapsulated or coated to NPs for developing novel nanocombinations with controlled surface characteristics. The development of approaches which might enhance conformational stabilization for several weeks of LPO via nanoformulation could improve the biopharmaceutical applicability of this bioactive ingredient. Nanoformulation of LPO enhances novel functions that can be useful in many biotechnological applications like food industry, cosmetic and pharmaceutical applications or to deliver and encapsulate bioactive components.