Protocol to characterize target components of Gelsemium using an in-house herb database and high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry.
Protocol to characterize target components of Gelsemium using an in-house herb database and high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry.
复制标题
DOI：
10.1016/j.xpro.2023.102510
复制
发表时间：
2023-09-15
期刊：
影响因子：
--
通讯作者：
Liu, Zhao-Ying
中科院分区：
文献类型：
--
作者：
Zuo, Meng-Ting;Liang, Ling-Ling;Gong, Meng-Die;Wang, Zi-Yuan;Liu, Zhao-Ying
目前，由于缺乏明确的标准，草药代谢物的鉴定具有挑战性。在这里，使用钩吻作为一个例子，我们提出了一个协议，用于表征目标成分的草药。该方法利用高效液相色谱/四极杆飞行时间质谱法，由基于报告研究和质谱法的内部草药代谢物数据库指导。我们描述了创建内部数据库，制备和检测样品，处理数据和表征化合物的步骤。该方法为今后中药代谢物的鉴定研究提供了参考。有关本方案使用和执行的完整详细信息，请参见Liu等人。（2017年）。通过建立内部代谢物数据库鉴定草药代谢物的方案样品制备、HPLC和MS操作说明制备钩吻样品提取MS活性成分的详细步骤从MS数据中处理和表征代谢物的步骤出版商说明：进行任何实验方案都需要遵守当地机构的实验室安全和伦理指南。目前，由于缺乏明确的标准，草药代谢物的鉴定具有挑战性。在这里，使用钩吻作为一个例子，我们提出了一个协议，用于表征目标成分的草药。该方法利用高效液相色谱/四极杆飞行时间质谱法，由基于报告研究和质谱法的内部草药代谢物数据库指导。我们描述了创建内部数据库，制备和检测样品，处理数据和表征化合物的步骤。该方法为今后中药代谢物的鉴定研究提供了参考。
Currently, the identification of herb metabolites is challenging due to a lack of clear standards. Here, using Gelsemium as an example, we present a protocol for characterizing target components of herbs. This approach utilizes high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry guided by an in-house herb metabolite database based on reported studies and mass spectrometry. We describe steps for creating an in-house database, preparing and detecting samples, processing data, and characterizing compounds. This approach offers a reference for future research on the identification of herb metabolites. For complete details on the use and execution of this protocol, please refer to Liu et al. (2017). Protocol to identify herb metabolites by building an in-house metabolite database Instructions to prepare samples, perform HPLC and MS Detailed steps to prepare Gelsemium sample to extract active ingredients for MS Steps to process and characterize metabolites from MS data Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Currently, the identification of herb metabolites is challenging due to a lack of clear standards. Here, using Gelsemium as an example, we present a protocol for characterizing target components of herbs. This approach utilizes high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry guided by an in-house herb metabolite database based on reported studies and mass spectrometry. We describe steps for creating an in-house database, preparing and detecting samples, processing data, and characterizing compounds. This approach offers a reference for future research on the identification of herb metabolites.
影响因子：
2.3
作者：
Liu, Yan-Chun;Xiao, Sa;Liu, Zhao-Ying
通讯作者：
Liu, Zhao-Ying