Infection Characteristics of Rice Stripe Mosaic Virus in the Body of the Vector Leafhoppers
Infection Characteristics of Rice Stripe Mosaic Virus in the Body of the Vector Leafhoppers
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水稻条纹花叶病毒在媒介叶蝉体内的感染特性
DOI：
10.3389/fmicb.2018.03258
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发表时间：
2019
影响因子：
5.2
通讯作者：
Jia Dongsheng
中科院分区：
文献类型：
--
作者：
Zhao Ping;Sun Xiang;Li Pan;Sun Jiatao;Yue Yue;Wei Jing;Wei Taiyun;Jia Dongsheng
水稻条纹花叶病毒（RSMV）是一种新的细胞habdovirus，由背叶蝉（Recilia dorsalis）以持续繁殖方式传播。在本研究中，我们首次证实了RSMV的N蛋白是dorsalis载体培养细胞中病毒质和病毒粒子的组成部分。共聚焦显微镜显示，RSMV最初积聚在背背鼠滤室的上皮细胞中，从那里扩散到滤室周围的内脏肌肉。随后，RSMV迅速通过悬韧带扩散到唾液腺。同时，RSMV从滤腔扩散到中肠、后肠、食道、血淋巴和中枢神经系统。我们进一步观察到RSMV颗粒在不同组织的细胞质中繁殖时呈非包膜形态，在昆虫体内扩散时呈包膜形态。此外，我们还发现绿叶蝉（Nephotettix virescens）也能够获得和传播RSMV。这些结果阐明了RSMV在其叶蝉载体中的感染特征，有助于指导RSMV防控策略的制定。
Rice stripe mosaic virus (RSMV), a novel species of Cytorhabdovirus, is transmitted by the leafhopper Recilia dorsalis in a persistent-propagative manner. In this study, we firstly confirmed that N protein of RSMV is a component of viroplasm and virion in vector culture cells of R. dorsalis. Confocal microscopy revealed that RSMV initially accumulated in epithelial cells of the filter chamber of R. dorsalis, from where it proceeded to the visceral muscles surrounding the filter chamber. Subsequently, RSMV spread quickly throughout the suspensory ligament to the salivary glands. Meanwhile, RSMV spread from the filter chamber to midgut, hindgut, esophagus, hemolymph, and central nervous system. We further observed that RSMV particles displayed as non-enveloped form when propagating in cytoplasm of different tissues, and became enveloped when spread within insect body by electron microscopy. Additionally, we found that the leafhopper Nephotettix virescens was also able to acquire and transmit RSMV. These results clarified the infection characteristics of RSMV in its leafhopper vectors, which will help guide the formulation of RSMV prevention and control strategies.