A UDP-HexNAc:Polyprenol-P GalNAc-1-P Transferase (WecP) Representing a New Subgroup of the Enzyme Family
A UDP-HexNAc:Polyprenol-P GalNAc-1-P Transferase (WecP) Representing a New Subgroup of the Enzyme Family
复制标题
DOI：
10.1128/jb.01441-10
复制
发表时间：
2011-04-01
影响因子：
3.2
通讯作者：
Tomas, Juan M.
中科院分区：
文献类型：
--
作者：
Merino, Susana;Jimenez, Natalia;Tomas, Juan M.
The Aeromonas hydrophila AH-3 WecP represents a new class of UDP-HexNAc:polyprenol-P HexNAc-1-P transferases. These enzymes use a membrane-associated polyprenol phosphate acceptor (undecaprenyl phosphate [Und-P]) and a cytoplasmic UDP-D-N-acetylhexosamine sugar nucleotide as the donor substrate. Until now, all the WecA enzymes tested were able to transfer UDP-GlcNAc to the Und-P. In this study, we present in vitro and in vivo proofs that A. hydrophila AH-3 WecP transfers GalNAc to Und-P and is unable to transfer GlcNAc to the same enzyme substrate. The molecular topology of WecP is more similar to that of WbaP (UDP-Gal polyprenol-P transferase) than to that of WecA (UDP-GlcNAc polyprenol-P transferase). WecP is the first UDP-HexNAc: polyprenol-P GalNAc-1-P transferase described.
