Rodent model of chronic central pain after spinal cord contusion injury and effects of gabapentin
Rodent model of chronic central pain after spinal cord contusion injury and effects of gabapentin
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DOI：
10.1089/neu.2000.17.1205
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发表时间：
2000-12-01
影响因子：
4.2
通讯作者：
McAdoo, DJ
中科院分区：
文献类型：
--
作者：
Hulsebosch, CE;Xu, GY;McAdoo, DJ
脊髓损伤（SCI）常导致患者出现异常疼痛综合征。我们提出了一种最近开发的慢性中枢性疼痛的SCI哺乳动物模型，其中使用纽约大学撞击器装置（10 g棒，2，0 mm直径，12，5 mm跌落高度）在T8处挫伤脊髓，该损伤的特征在于行为上为中度。运动功能的恢复通过旷场试验进行评估，并使用旷场试验量表（BBB量表）进行评分。进行了对机械点状（von Frey毛）和非点状（4 mm直径钝头探针）以及热（辐射热）外周刺激的缩爪反应伴随脊髓上反应的体感测试。在个体动物水平上比较挫伤前和挫伤后的反应表明，对低阈值点状机械刺激、非点状刺激和热刺激的反应显著增加（p <0.05）。为了证明该模型作为中枢性疼痛模型的有效性，将加巴喷丁（临床上用于中枢性疼痛的药剂）以10或30 mg/kg腹膜内给予，加巴喷丁治疗显著且可逆地改变了这些反应，与异常感觉行为的衰减一致，并且衰减的反应持续药物作用的持续时间（长达6 h）。这些结果支持在SCI后慢性中枢性疼痛的研究中使用脊髓挫伤模型。
Spinal cord injury (SCI) often results in abnormal pain syndromes in patients. We present a recently developed SCI mammalian model of chronic central pain in which the spinal cord is contused at T8 using the NYU impactor device (10-g rod, 2,0-mm diameter, 12,5-mm drop height), an injury which is characterized behaviorally as moderate. Recovery of locomotor function was assessed with an open field test and scored using the open field test scale (BBB scale). Somatosensory tests of paw withdrawal responses accompanied by supraspinal responses to both mechanical punctate (von Frey hairs) and nonpunctate (4 mm diameter blunt probe) as well as thermal (radiant heat) peripheral stimuli were performed. Comparisons at the level of the individual animal between precontusion and postcontusiom responses indicated significant increases in reactions to low threshold punctate mechanical stimuli, non-punctate stimuli and thermal stimuli (p < 0,05), To demonstrate the validity of this model as a central pain model, gabapentin, an agent used clinically for central pain, was given i.p, at 10 or 30 mg/kg, Gabapentin treatment significantly and reversibly changed the responses, consistent with the attenuation of the abnormal sensory behavior, and the attenuated responses lasted for the duration of the drug effect (up to 6 h), These results support the use of the spinal contusion model in the study of chronic central pain after SCI.