Immunohistochemical comparison of astrocytic mGluR5 upregulation in infraorbital nerve-versus sciatic nerve-ligated rat
Immunohistochemical comparison of astrocytic mGluR5 upregulation in infraorbital nerve-versus sciatic nerve-ligated rat
复制标题
DOI：
10.1016/j.neulet.2017.05.035
复制
发表时间：
2017-07-13
影响因子：
2.5
通讯作者：
Hermans, Emmanuel
中科院分区：
文献类型：
--
作者：
Michot, Benoit;Deumens, Ronald;Hermans, Emmanuel
头部和头外神经病理性疼痛的不同药理反应被认为与不同的机制有关，可能涉及神经胶质细胞介导的神经炎性反应。星形胶质细胞对神经病理性疼痛的神经元敏化特别重要，这部分是通过调节谷氨酸能传递来实现的。由于代谢性谷氨酸受体5(MGluR5)参与了谷氨酸能系统的星形细胞调节，我们研究了其在头痛性和头外神经病理性疼痛模型中表达的变化。成年雄性大鼠造成一侧眶下神经(ION)或坐骨神经(SN)慢性压迫损伤(CCI)。7d后，mGluR5和星形胶质细胞标记物GFAP(胶质纤维酸性蛋白)在CCI-SN大鼠的三叉神经核浅层和脊髓背角均有表达。此外，GFAP和mGluR5的共存强烈表明，与假手术组相比，神经损伤大鼠星形胶质细胞mGluR5的表达增加。目前的数据显示，在CCI-ion和CCI-SN中，星形细胞mGIuR5在中央结构中上调。这提示，mGIuR5的药理调节可能是一种减轻头内外神经病理性疼痛的新途径。(C)2017爱思唯尔B.V.保留所有权利。
The differential pharmacological responsiveness of cephalic and extra-cephalic neuropathic pain has been proposed to relate to distinct mechanisms that may involve neuroinflammatory reactions mediated by glial cells. Astrocytes are particularly important for neuronal sensitization in neuropathic pain, in part through modulation of glutamatergic transmission. Because the metabotropic glutamate receptor 5 (mGluR5) is involved in the astrocytic regulation of the glutamatergic system, we investigated modifications of its expression in models of cephalic versus extra-cephalic neuropathic pain. Adult male rats underwent unilateral chronic constriction injury (CCI) of either the infraorbital nerve (ION) or the sciatic nerve (SN). Seven days later, mGluR5 and the astrocyte marker GFAP (glial fibrillary acidic protein) were overexpressed and appeared localized mainly in the superficial lamina of the trigeminal nucleus in CCI-ION and the spinal cord dorsal horn in CCI-SN rats. In addition, colocalization of GFAP and mGluR5 strongly suggested an increase of astrocytic mGluR5 expression in nerve-injured rats compared to sham animals. The present data show an upregulation of astrocytic mGIuR5 in central structures in both CCI-ION and CCI-SN. This suggests that the pharmacological modulation of mGIuR5 could be a new approach to reduce both cephalic and extra-cephalic neuropathic pain. (C) 2017 Elsevier B.V. All rights reserved.