Functional expression of a microinjected Edα gene in C57BL/6 transgenic mice
Functional expression of a microinjected Edα gene in C57BL/6 transgenic mice
复制标题
显微注射 Edα 基因在 C57BL/6 转基因小鼠中的功能表达
作者：
K. Yamamura;H. Kikutani;V. Folsom;L. Clayton;M. Kimoto;S. Akira;S. Kashiwamura;S. Tonegawa;T. Kishimoto
已在某些免疫活性细胞（包括B淋巴细胞和单核细胞）的表面检测到II类主要组织相容性抗原I-A和I-E 1 -3。这些分子参与免疫应答中的细胞-细胞相互作用4，5。每个II类抗原由两个亚基（α和β链）组成，编码这些亚基的基因已在分子水平上得到充分表征6 -9。为探讨Eα基因表达的调控机制及I-E抗原在免疫调节中的作用，我们将克隆的艾德α基因显微注射到B单倍型C57 BL/6小鼠的受精卵中，制备了转基因小鼠。该品系小鼠在覆盖转录启动子的Eα基因上游（5′）区域中携带缺失，因此不表达该基因10。有趣的是，Eα基因的这种遗传缺陷伴随着宿主小鼠无法对某组抗原做出反应，这种现象通常称为IR基因控制11，12。我们在此报告了艾德α基因在这些转基因小鼠中表达，在B淋巴细胞和单核细胞表面形成I-Edα艾德β抗原，这些I-E抗原在诱导混合淋巴细胞反应和恢复对聚（L-谷氨酸-L-赖氨酸-L-苯丙氨酸）（GL-Phe）的免疫应答方面具有功能。
The class II major histocompatibility antigens, I–A and I–E, have been detected on the surface of certain immunocompetent cells, including B lymphocytes and monocytes1–3. These molecules are involved in cell–cell interactions in the immune responses4,5. Each class II antigen consists of two subunits, α and β chains, and the genes encoding these subunits have been well characterized at the molecular level6–9. To analyse the regulatory mechanism of Eα gene expression and the role of the I–E antigen in the regulation of the immune responses, we have produced transgenic mice by microinjecting cloned Edα genes into fertilized eggs of C57BL/6 mice of b haplotype. This strain of mouse carries a deletion in the upstream (5′) region of the Eα gene covering the transcriptional promoter and, therefore, does not express this gene10. Interestingly, this genetic defect of the Eα gene is accompanied by the inability of the host mouse to respond to a certain set of antigens, phenomena generally termed Ir gene control11,12. We report here that the Edα genes are expressed in these transgenic mice to form the I–EdαEdβ antigen on the surface of B lymphocytes and monocytes and that these I–E antigens are functional in terms of the induction of a mixed lymphocyte reaction and the restoration of immune responsiveness to poly(L-glutamic acid-L-lysine-L-phenylalanine) (GL-Phe).
转染的小鼠 II 类主要组织相容性复合体基因的功能和诱导表达。
DOI：
10.1073/pnas.81.7.2045
发表时间：
1984
影响因子：
11.1
作者：
Folsom,V;Gold,DP;White,J;Marrack,P;Kappler,J;Tonegawa,S
通讯作者：
Tonegawa,S
DNA介导的基因转移后L细胞中H-2Dd和H-2Ld小鼠主要组织相容性抗原基因的表达。
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Margulies,DH;Evans,GA;Ozato,K;Camerini-Otero,RD;Tanaka,K;Appella,E;Seidman,JG
通讯作者：
Seidman,JG