Correlation between Genotypic Diversity, Lipooligosaccharide Gene Locus Class Variation, and Caco-2 Cell Invasion Potential of Campylobacter jejuni Isolates from Chicken Meat and Humans: Contribution to Virulotyping
Correlation between Genotypic Diversity, Lipooligosaccharide Gene Locus Class Variation, and Caco-2 Cell Invasion Potential of Campylobacter jejuni Isolates from Chicken Meat and Humans: Contribution to Virulotyping
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DOI：
10.1128/aem.02269-08
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发表时间：
2009-07-01
影响因子：
4.4
通讯作者：
De Zutter, Lieven
中科院分区：
文献类型：
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作者：
Habib, Ihab;Louwen, Rogier;De Zutter, Lieven
研究空肠弯曲菌脂寡糖（LOS）的重大兴趣源于其在感染后麻痹性疾病中的潜在作用。在这项研究中，我们提出了五个LOS位点类（A，B，C，D和E）的116 C收集PCR筛选的结果。来自鸡肉的空肠分离株（n = 76）和散发的人类腹泻病例（n = 40）。我们将LOS类别与多位点序列分型（MLST）分配的克隆复合体（CC）相关。最后，我们评估了一组52个这些C的入侵潜力。空肠分离物的Caco-2细胞。PCR筛选结果显示87.1%（101/116）的菌株可归属于LOS A、B、C、D或E类。LOS类和某些MLST CC之间的一致性被揭示。绝大多数（85.7% [24/28]）的C.分组在CC-21中的空肠分离株显示表达C类LOS基因座。C. Caco-2细胞的唾液酸化LOS（A、B和C类）的空肠分离株（n = 29）显著高于C.非唾液酸化LOS的空肠分离株（n = 23; D类和E类）。鸡肉分离株和人分离株的侵袭力无显著差异。然而，C.分配到CC-206（与LOS等级B相关）或CC-21（与LOS等级C相关）的空肠分离株显示统计学上显著高于来自其它CC的分离株的侵袭水平。LOS类和CC之间的相关性进一步证实了脉冲场凝胶电泳。本研究揭示了C.空肠。结果表明，简单PCR筛查C.空肠LOS分类可以可靠地预测某些MLST CC，并增加分子分型结果的解释。我们的研究证实了LOS的唾液酸化对C.空肠适合度和毒力。细胞表面糖结构的改变可以增强C.空肠适应宿主或在宿主中生存。
Significant interest in studying the lipooligosaccharide (LOS) of Campylobacter jejuni has stemmed from its potential role in postinfection paralytic disorders. In this study we present the results of PCR screening of five LOS locus classes (A, B, C, D, and E) for a collection of 116 C. jejuni isolates from chicken meat (n = 76) and sporadic human cases of diarrhea (n = 40). We correlated LOS classes with clonal complexes (CC) assigned by multilocus sequence typing (MLST). Finally, we evaluated the invasion potential of a panel of 52 of these C. jejuni isolates for Caco-2 cells. PCR screening showed that 87.1% (101/116) of isolates could be assigned to LOS class A, B, C, D, or E. Concordance between LOS classes and certain MLST CC was revealed. The majority (85.7% [24/28]) of C. jejuni isolates grouped in CC-21 were shown to express LOS locus class C. The invasion potential of C. jejuni isolates possessing sialylated LOS (n = 29; classes A, B, and C) for Caco-2 cells was significantly higher (P < 0.0001) than that of C. jejuni isolates with nonsialylated LOS (n = 23; classes D and E). There was no significant difference in invasiveness between chicken meat and human isolates. However, C. jejuni isolates assigned to CC-206 (correlated with LOS class B) or CC-21 (correlated with LOS class C) showed statistically significantly higher levels of invasion than isolates from other CC. Correlation between LOS classes and CC was further confirmed by pulsed-field gel electrophoresis. The present study reveals a correlation between genotypic diversity and LOS locus classes of C. jejuni. We showed that simple PCR screening for C. jejuni LOS classes could reliably predict certain MLST CC and add to the interpretation of molecular-typing results. Our study corroborates that sialylation of LOS is advantageous for C. jejuni fitness and virulence in different hosts. The modulation of cell surface carbohydrate structure could enhance the ability of C. jejuni to adapt to or survive in a host.