Identification and characterization of a novel Channelrhodopsin gene HpChR1 in Haematococcus pluvialis
Identification and characterization of a novel Channelrhodopsin gene HpChR1 in Haematococcus pluvialis
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雨生红球藻中新型视紫红质通道蛋白基因 HpChR1 的鉴定和表征
DOI：
10.1016/j.algal.2021.102263
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发表时间：
2021-05
影响因子：
5.1
通讯作者：
Zhangli Hu
中科院分区：
文献类型：
--
作者：
Ming Tao;Kang Xiao;Yihong Zheng;Li Ze;Qiulan Luo;Guanbo Wang;Zhangli Hu
摘要工业用雨生红球藻暴露于一定波长的光中，可促进虾青素的生物合成。光响应性生物合成过程可能是由光感受器蛋白介导的。因此，迫切需要研究特别负责藻类生长和代谢物合成的光感受器。克隆了一个新的通道视紫红质基因HpChR1。我们通过活细胞成像检测了HpChR1在HeLa细胞中的亚细胞定位和细胞功能。HpChR1主要定位于HeLa细胞的内质网和胞核，并介导内质网钙释放。HpChR1蛋白在N端有7个跨膜结构域，在C端有一个可能的MED15(介体复合体15)结构域。通过酵母双杂交，我们显示了HpChR1的结合伙伴，并进一步帮助解释了HpChR1的功能。这些结果表明，HpChR1可能作为通道视紫红质参与了光子能量和信号的接收和转换，这可能为理解雨生红豆杉的光响应性生物合成机制奠定基础。
Abstract Exposure to light with certain wavelengths was demonstrated to enhance the biosynthesis of astaxanthin in Haematococcus pluvialis for industrial purposes. Photo-responsive biosynthetic process is likely to be mediated by photoreceptor proteins. Therefore, the study of photoreceptors particularly responsible for algal growth and metabolite synthesis is in urgent demand. A novel channelrhodopsin gene HpChR1 was cloned. We examined the subcellular localization and cellular functions of HpChR1 in HeLa cells by living cell imaging. HpChR1 was shown to mainly be localized to the endoplasmic reticulum and the nucleus and mediate endoplasmic reticulum calcium release in HeLa cells. HpChR1 protein comprises seven transmembrane domains at N-terminus and a putative MED15 (mediator complex subunit 15) domain at C-terminus. By using Yeast Two-Hybrid, we showed the binding partners of HpChR1 and they further assisting to interpret the functional roles of HpChR1.These results showed HpChR1 may involve in the reception and transformation of photon energy and signal as channelrhodopsin, and this may shed light on understanding photo-responsive biosynthetic mechanisms in H. pluvialis.
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影响因子：
64.8
作者：
Kim YS;Kato HE;Yamashita K;Ito S;Inoue K;Ramakrishnan C;Fenno LE;Evans KE;Paggi JM;Dror RO;Kandori H;Kobilka BK;Deisseroth K
通讯作者：
Deisseroth K
影响因子：
56.9
作者：
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通讯作者：
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影响因子：
25
作者：
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通讯作者：
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影响因子：
11.4
作者：
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通讯作者：
Zhang, Weiwen
影响因子：
9.2
作者：
Nagel, G;Brauner, M;Gottschalk, A
通讯作者：
Gottschalk, A