Pectinase-responsive carriers based on mesoporous silica nanoparticles for improving the translocation and fungicidal activity of prochloraz in rice plants
Pectinase-responsive carriers based on mesoporous silica nanoparticles for improving the translocation and fungicidal activity of prochloraz in rice plants
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DOI：
10.1016/j.cej.2020.126440
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发表时间：
2021-01-15
影响因子：
15.1
通讯作者：
He, Shun
中科院分区：
文献类型：
--
作者：
Abdelrahman, Talat M.;Qin, Xueying;He, Shun
刺激响应型载体作为农药向植物体内释放的传递系统，可以提高农药对靶标害虫的利用效率，减少对非靶标生物和环境的危害。将咪鲜胺负载于介孔二氧化硅纳米粒子（MSNs）中，并在粒子表面包覆果胶作为"看门人“，研究咪鲜胺@ MSN-Pec（Pro@ MSN-Pec）在水稻植株中的转运和分布。结果表明，成功制备了Pro@MSN-Pec，其平均粒径为70.89 nm，负载率为30% w/w。用异硫氰酸荧光素（FITC）标记MSNs，以跟踪载体在水稻植株中的分布。共聚焦扫描分析表明，MSN-FITC可以从处理的叶片或根运输到水稻的所有部位。咪鲜胺（Pro）从Pro@MSN-Pec中的释放是果胶酶依赖性的。与市售的Pro乳油（EC）相比，Pro@MSN-Pec配方在水稻植株中的吸收和转运能力更强，对稻瘟病的持效期更长，抑菌效果更好。Pro在水稻植株中的最终残留量低于最大残留限量。这些结果表明，在植物中使用刺激响应载体作为农药递送系统可以潜在地在应用农业中实施。
Stimuli-responsive carriers as delivery systems of agrochemicals into plants can increase the utilization efficiency of pesticides against target pests and reduce hazards on nontarget living organisms and the environment. In the present work, prochloraz was loaded into mesoporous silica nanoparticles (MSNs) and coated by pectin on the particle surface as a gatekeeper to study the translocation and distribution of prochloraz@MSN-pectin (Pro@ MSN-Pec) in rice plants. The results showed that Pro@MSN-Pec was successfully fabricated and had an average size of 70.89 nm and a good loading efficiency (30% w/w). The MSNs were labeled using fluorescein isothiocyanate (FITC) to track the distribution of the carriers in the rice plants. Confocal scanning analysis showed that MSN-FITC could be transported to all rice parts from the treated leaves or roots. The release of prochloraz (Pro) from Pro@MSN-Pec was pectinase-dependent. Compared with the commercial emulsifiable concentrate (EC formulation) of Pro, the Pro@MSN-Pec formula had a better uptake and translocation in rice plants as well as a longer duration and better antifungal activity against rice blast disease (Magnaporthe oryzae). The final residue level of Pro in the rice plants was lower than the maximum residue limits. These results suggest that the use of stimuli-responsive carriers as pesticide-delivery systems in plants can be potentially implemented in applied agriculture.