DIFFERENT OUABAIN SENSITIVITIES OF NA+/K+-ATPASE FROM POEKILOCERUS-BUFONIUS TISSUES AND A POSSIBLE PHYSIOLOGICAL COST
DIFFERENT OUABAIN SENSITIVITIES OF NA+/K+-ATPASE FROM POEKILOCERUS-BUFONIUS TISSUES AND A POSSIBLE PHYSIOLOGICAL COST
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DOI：
10.1016/0305-0491(93)90034-3
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发表时间：
1993-12-01
影响因子：
2.2
通讯作者：
ALROBAI, AA
中科院分区：
文献类型：
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作者：
ALROBAI, AA
1.研究了中华蚱蜢（Poekilocerusbufonius）中肠微粒体Na+/K+转运ATP酶的性质，并与脑和排泄系统中的相同酶进行了比较.在所研究的三种组织中存在两种ATP酶活性组分.中肠、脑和排泄系统（后肠+马氏管）Na+/K+转运ATP酶的理化性质基本相同.中肠、排泄系统和脑Na+/K+-ATP酶的PI 50计算值分别为2（I50 = 1 × 10（-2）M）、3.7（I50 = 2 × 10（-4）M）和6.4（I50 = 3.98 × 10（-7））。中肠含有最多的哇巴因抗性Na+/K+-ATP酶。结果表明，蟾蜍对有毒强心苷（CGS）产生了一定的耐受性，但脑组织中存在哇巴因敏感的ATP酶，表明存在自毒性的可能性。6.因此，蟾毒实蝇不同组织中Na+/K+-ATP酶的差异可能是由于同一昆虫中哇巴因敏感性（或同工酶谱）的组织依赖性差异所致.蟾蜍雌蝇飞行肌萎缩的现象表明，昆虫取食有毒植物可能会造成生理代价。
1. The properties of Na+/K+-transporting ATPase in microsomal preparation from mid-gut of the grasshopper, Poekilocerus bufonius, were investigated and compared with the same enzyme from brain and excretory system.2. Two components of ATPases activity are present in the three tissues studied.3. The physiochemical properties of Na+/K+-transporting ATPase from mid-gut, brain and excretory system (hind-gut plus Malpighian tubules) are essentially the same.4. The calculated values of PI50 were 2 (I50 = 1 x 10(-2) M), 3.7 (I50 = 2 x 10(-4) M) and 6.4 (I50 = 3.98 x 10(-7)) for Na+/K+-ATPase from mid-gut, excretory system and brain, respectively. The mid-gut contains the most ouabain-resistant Na+/K+-ATPase.5. The results suggest that P. bufonius have developed some tolerance to toxic cardiac glycosides (CGS), but there is a possibility of autotoxicity as indicated by the presence of ouabain-sensitive ATPase from brain tissue.6. It was concluded that the dissimilarities of Na+/K+-ATPases from different tissues of P. bufonius are probably due to tissue-dependent differences in ouabain sensitivity (or isoenzymes pattern) available in the same insect.7. The atrophy of female flight muscle of P. bufonius suggests the possibility of physiological cost inflicted on insects consuming poisonous plants.