Organization of the rat Tage4 gene and herpesvirus entry activity of the encoded protein
Organization of the rat Tage4 gene and herpesvirus entry activity of the encoded protein
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DOI：
10.1016/s0378-1119(01)00343-2
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发表时间：
2001-03-07
期刊：
影响因子：
3.5
通讯作者：
Denis, MG
中科院分区：
文献类型：
--
作者：
Baury, B;Geraghty, RJ;Denis, MG
Tage4 基因（肿瘤相关糖蛋白 E4）是免疫球蛋白超家族的成员，在大鼠结肠肿瘤和 Min 小鼠肠腺瘤中过度表达。 Tage4 cDNA 与人 CD155 具有 60% 的同一性，CD155 是免疫球蛋白超家族的成员，编码跨膜蛋白，能够作为脊髓灰质炎病毒、猪伪狂犬病病毒和牛疱疹病毒 1 的进入受体。我们确定了 Tage4 基因的结构。该基因大约有15 kb，由8个外显子和7个内含子组成。我们还分离了 Tage4 基因 5' 侧翼区域约 2 kb 的区域，并证明了紧密聚集的转录起始位点的存在。 RT-PCR 未鉴定出剪接变体，表明 Tage4 基因被转录为独特的 mRNA。最后，测试了 Tage4 基因编码的蛋白质介导多种病毒进入的能力。将大鼠 Tage4 基因的这些结构和功能特征与人类 CD155 基因的结构和功能特征进行了比较。结果表明Tage4基因可能与CD155基因是直系同源的。 (C) 2001 Elsevier Science B.V. 保留所有权利。
The Tage4 gene (Tumor-Associated Glycoprotein E4) is a member of the immunoglobulin superfamily overexpressed in rat colon tumors and Min mouse intestinal adenomas. The Tage4 cDNA presents similar to 60% identity with the human CD155, a member of the immunoglobulin superfamily coding for a transmembrane protein capable of serving as an entry receptor for poliovirus, porcine pseudorabies virus and bovine herpesvirus 1. We determined the structure of the Tage4 gene. This gene covers similar to 15 kb and is composed of eight exons and seven introns. We also isolated similar to2 kb of the 5' flanking region of the Tage4 gene and demonstrated the existence of closely clustered transcription start sites. No splicing variant was identified by RT-PCR indicating that the Tage4 gene is transcribed as a unique mRNA. Finally, the protein encoded by the Tage4 gene was tested for ability to mediate entry of several viruses. These structural and functional features of the rat Tage4 gene were compared to those of the human CD155 gene. The results indicated that the Tage4 gene is probably orthologous to the gene for CD155. (C) 2001 Elsevier Science B.V. All rights reserved.