Plants recruit insecticidal bacteria to defend against herbivore attacks
Plants recruit insecticidal bacteria to defend against herbivore attacks
复制标题
植物招募杀虫细菌来防御食草动物的攻击
DOI：
10.1016/j.micres.2023.127597
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发表时间：
2024-01-23
影响因子：
6.7
通讯作者：
Geng，Lili
中科院分区：
文献类型：
--
作者：
Xu，Wenyu;Sun，Xiaoxiao;Geng，Lili
害虫取食影响根际细菌群落。根际微生物群激活水杨酸和茉莉酸信号通路，帮助植物应对害虫侵扰。然而，植物是否能够招募特殊的杀虫微生物来应对食草动物的攻击尚不清楚。利用花生和平行黑鳃金龟一龄幼虫组成的系统，分析花生在幼虫取食后是否真正富集了杀虫细菌，以及接种富集的细菌是否促进了植物对草食动物的抗性。本研究利用16S rRNA基因扩增子的高通量测序来证明地下害虫黑鳃金龟的侵染。平行24小时内快速改变根际细菌群落结构，肠杆菌科，特别是肠杆菌丰度明显富集。根部取食诱导根际细菌形成比对照更复杂的共生网络。分离得到根际细菌，其中4株对幽门螺旋杆菌具有高毒力。通过随机森林分析获得平行体。在使用分根系统的回接种实验中，绿色荧光蛋白（GFP）标记的肠杆菌属。观察到 IPPBiotE33 在未食用的花生根中富集。此外，补充IPPBiotE33可减轻幽门螺杆菌的不利影响。平行花生。我们的研究结果表明，食草动物的侵扰可以诱导植物组装具有特定杀幼虫活性的细菌来应对威胁。
Pest feeding affects the rhizobacteria community. The rhizomicrobiota activates salicylic acid and jasmonic acid signaling pathways to help plants deal with pest infestation. However, whether plants can recruit special pesticidal microorganisms to deal with attack from herbivores is unclear. A system composed of peanuts and first-instar larvae ofHolotrichia parallelawere used to analyze whether peanuts truly enrich the insecticidal bacteria after feeding by larvae, and whether inoculation of the enriched bacteria promotes the resistance of plants to herbivore.In this study, high-throughput sequencing of 16 S rRNA gene amplicons was used to demonstrate that infestation of the subterranean pestH. parallelaquickly changed the rhizosphere bacterial community structure within 24 h, and the abundance of Enterobacteriaceae, especially Enterobacter, was manifestly enriched. Root feeding induced rhizobacteria to form a more complex co-occurrence network than the control. Rhizosphere bacteria were isolated, and 4 isolates with high toxicity againstH. parallelalarvae were obtained by random forest analysis. In a back-inoculation experiment using a split-root system, green fluorescent protein (GFP)-labeledEnterobactersp. IPPBiotE33 was observed to be enriched in uneaten peanut roots. Additionally, supplementation with IPPBiotE33 alleviated the adverse effects ofH. parallelaon peanuts. Our findings indicated that herbivore infestation could induce plants to assemble bacteria with specific larvicidal activity to address threats.