Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells.

Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells.
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DOI:
10.1007/978-1-4939-7116-9_18
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发表时间:
2017
影响因子:
--
通讯作者:
Judith Olejnik;E. Nelson
Judith Olejnik;E. Nelson
中科院分区:
--
文献类型:
--
作者:
Judith Olejnik;E. Nelson

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In this chapter, we describe a Western blot assay for the successful detection of apoptosis in Ebola virus (EBOV)-infected cells. The protocol includes all steps from cell culture, infection of cells, generation of lysates, and analysis using Western blot to detect caspase cleavage as marker of apoptosis.