Novel Polysaccharide-Derived Hydrogel Prevents Perineural Adhesions in a Rat Model of Sciatic Nerve Adhesion
Novel Polysaccharide-Derived Hydrogel Prevents Perineural Adhesions in a Rat Model of Sciatic Nerve Adhesion
复制标题
DOI：
10.1002/jor.21004
复制
发表时间：
2010-03-01
影响因子：
2.8
通讯作者：
Hirata, Hitoshi
中科院分区：
文献类型：
--
作者：
Yamamoto, Michiro;Endo, Nobuyuki;Hirata, Hitoshi
我们研究了一种新的羧甲基纤维素（CMC）衍生的水凝胶，其中磷脂酰乙醇胺（PE）被引入到CMC的羧基中，用于防止大鼠坐骨神经广泛内神经松解后的神经周围粘连。将坐骨神经随机分配到以下组之一：对照组，手术但不治疗，IIA组，手术并用1%透明质酸治疗，CMC-PE（L）组，手术后用低粘度CMC-PE水凝胶治疗，CMC-PE（H）组，用高粘度CMC-PE水凝胶进行手术和治疗，在6周时评价神经周围粘连。还对神经进行生物力学测试以评估极限断裂强度。进行电生理和湿肌肉重量测量。在20天时，对照组的潜伏期显著长于CMC-PE（L）组。在6周时，对照组的湿肌肉重量与体重的平均百分比显著低于CMC-PE（L）组。低粘度CMC-PE水凝胶似乎可以预防神经周围粘连并允许神经功能的早期恢复2009年由Wiley Periodicals，Inc.出版的骨科研究学会J Orthop Res 28 284-288，2010
We investigated the effects of a novel carboxymethylcellulose (CMC)-derived hydrogel, in which phosphatidylethanolamine (PE) was introduced into the carboxyl groups of CMC, for preventing perineural adhesion after extensive internal neurolysis of rat sciatic nerve Sciatic nerves were randomly assigned to one of the following groups the Control group, operated but no treatment, the IIA group, operated and treated with 1% hyaluronan, the CMC-PE(L) group, operated and treated with low-viscosity CMC-PE hydrogel, and the CMC-PE(H) group, operated and treated with high-viscosity CMC-PE hydrogel Perineural adhesions were evaluated at 6 weeks Nerves were also subjected to biomechanical testing to assess ultimate breaking strength Electrophysiological and wet muscle weight measurements were performed Breaking strengths were significantly lower for the CMC-PE(L) group than for the Control and HA groups Latency was significant longer for the Control group than of the CMC-PE(L) group at 20 days. The mean percentage of wet muscle weight to body weight was significantly lower for the Control group than for the CMC-PE(L) group at 6 weeks Low-viscosity CMC-PE hydrogel appears to prevent perineural adhesions and allow early restoration of nerve function 2009 Orthopaedic Research Society Published by Wiley Periodicals, Inc J Orthop Res 28 284-288, 2010