Immunohistochemical and immunoelectron microscopic localization of S-100 protein in the interdigitating reticulum cells of the human lymph node
Immunohistochemical and immunoelectron microscopic localization of S-100 protein in the interdigitating reticulum cells of the human lymph node
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人淋巴结叉指网状细胞中 S-100 蛋白的免疫组织化学和免疫电镜定位
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
Y. Nakazato
中科院分区：
文献类型：
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作者：
Kiyoshi Takahashi;H. Yamaguchi;J. Ishizeki;T. Nakajima;Y. Nakazato
本文应用过氧化物酶抗过氧化物酶（PAP）法和免疫电镜技术，对5例非特异性淋巴结炎和4例皮肤病性淋巴结炎的淋巴结进行了观察。在非特异性淋巴结炎胸腺依赖区的树突状细胞中检测到S-100蛋白。皮肤病性淋巴结炎的副皮质区显示含S-100蛋白的树突状细胞的显著积聚。免疫电镜显示，非特异性和皮肤病性淋巴结炎的含S-100蛋白的树突状细胞对应于交错网状细胞（IRC）。S-100蛋白在细胞核、细胞质和核膜上均有表达，提示IRCs自身合成S-100蛋白。S-100蛋白是一个非常有用的细胞标记物，用于识别淋巴组织中的IRC。
SummaryLymph nodes of 5 cases with non-specific lymphadenitis and of 4 cases with dermatopathic lymphadenitis were examined by the peroxidase anti-peroxidase (PAP) method and by immunoelectron microscopy using monospecific antibody against nervous system specific S-100 protein. S-100 protein was detected in dendritic shaped cells of the thymus-dependent area of non-specific lymphadenitis. The paracortical area of dermatopathic lymphadenitis showed a marked accumulation of S-100 protein-containing dendritic shaped cells. Immunoelectron micrographs revealed that S-100 proteincontaining d endritic shaped cells of both non-specific and dermatopathic lymphadenitis corresponded to interdigitating reticulum cells (IRC). The detection of S-100 protein in the nucleus and on the cytoplasmic and nuclear membrane suggested that IRCs themselves synthesized S-100 protein. S-100 protein is a very useful cell marker for the identification of IRC in the lymphoid tissue.