Effect of of ATP on Neurons of the Rat Intact Nodose Ganglion
Effect of of ATP on Neurons of the Rat Intact Nodose Ganglion
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DOI：
10.1007/s11062-012-9247-3
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发表时间：
2012-03-01
期刊：
影响因子：
0.5
通讯作者：
Krishtal, O. A.
中科院分区：
文献类型：
--
作者：
Bobryshev, A. Yu.;Petrushenko, E. A.;Krishtal, O. A.
在细胞内记录下，我们研究了ATP对大鼠完整结状神经节神经细胞的作用。检查的神经元的静息膜电位平均为-60.3 +/- 1.4 mV（n = 84）;在这些单位中，88%被归类为C细胞。使用改良的层流系统将2 mM ATP局部应用于神经节表面导致神经元平均去极化7.1垂直于0.9 mV（n = 19）。P2 X受体的阻断剂PPADS（100 μ M）抑制这些去极化反应，使其幅度平均降低至初始值的16 +/- 3%（n = 3）。所获得的数据表明，绝大多数的完整的结状神经节的神经元具有功能的P2 X受体在其膜上。在完整脊神经节的相当一部分神经元中没有相应的反应[13-15]，显然是由以下事实确定的：在所述实验过程中，P2 X受体在ATP达到有效浓度之前有足够的时间脱敏。
Under intracellular recording, we studied the effect of ATP on nerve cells of the rat intact nodose ganglion. The resting membrane potential of the examined neurons was, on average, -60.3 +/- 1.4 mV (n = 84); among such units, 88% were classified as C cells. Local application of 2 mM ATP to the surface of the ganglion using a modified laminar flow system led to depolarization of neurons by 7.1 perpendicular to 0.9 mV, on average (n = 19). A blocker of P2X receptors, PPADS (100 mu M), suppressed these depolarization responses, decreasing their amplitude, on average, to 16 +/- 3% (n = 3) of the initial value. The obtained data indicate that an overwhelming majority of neurons of the intact nodose ganglion possess functional P2X receptors on their membranes. The absence of the corresponding responses in a considerable part of neurons of intact spinal ganglia [13-15] was, apparently, determined by the fact that P2X receptors in the course of the described experiments had enough time to desensitize before ATP reached the effective concentration.